不同干燥工艺制备的金翘热毒清的抗流感病毒作用比较  

Anti-influenza Virus Activity of Jinqiao Reduqing Prepared by Different Drying Process

作　　者：罗亚君[1] 贺帅[1] 张忠义[1] 

机构地区：[1]南方医科大学珠江医院,广州510280

出　　处：《中国实验方剂学杂志》2015年第12期104-107,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广州市科技计划项目(2012Y2-00018-2);广州市海珠区科技计划项目(2011-YL-02)

摘　　要：目的:观察金翘热毒清颗粒和金翘热毒清喷雾干燥粉体内、外的抗流感病毒作用,并比较不同干燥工艺制备的金翘热毒清抗病毒作用差异。方法:体外采用四氮唑蓝(MTT)比色法测定样品以不同给药方式(药物直接作用于病毒、药物直接作用于病毒感染的细胞)抑制甲型流感病毒鼠肺适应株(FM1)的半数抑制浓度(IC50),并计算治疗指数(TI)。狗肾细胞密度为1×104/孔,金翘热毒清喷雾干燥粉质量浓度为(2.61,1.31,0.66,0.16 g·L-1),金翘热毒清颗粒(6.01,3.05,1.52,0.76 g·L-1),作用时间为5 d,每组4个样本。将小鼠随机分为对照组,利巴韦林组(0.07 g·kg-1),金翘热毒清颗粒(按生药量计)组(11.7 g·kg-1)、金翘热毒清喷雾干燥粉(按生药量计)组(5.85,11.7,23.4 g·kg-1),采用流感病毒株FM1滴鼻感染小鼠建立感染模型,感染2 h后连续ig给药5 d,每天2次,以生命延长率和肺指数抑制率为指标,评价二者的保护作用。结果:在药物直接作用于病毒组和药物直接作用于病毒感染的细胞组中,金翘热毒清喷雾干燥粉的IC50分别为0.77,0.80 g·L-1,TI分别为11.78,11.34;金翘热毒清颗粒的IC50分别为3.70,3.60 g·L-1,TI分别为6.37,6.55;金翘热毒清喷雾干燥粉23.4 g·kg-1,其生命延长率为60.81%,肺指数抑制率为13.18%,与病毒组比较,差异均有统计学意义(P<0.05);金翘热毒清颗粒与金翘热毒清喷雾干燥粉相同剂量下的实验结果平行比较,未见显著差异。结论:金翘热毒清颗粒与金翘热毒清喷雾干燥粉都有抗流感病毒作用;体外在相同剂量下金翘热毒清喷雾干燥粉对流感病毒株的抑制作用优于金翘热毒清颗粒。Objective： To investigate antiviral activity of Jinqiao Reduqing granules and Jinqiao Reduqing spray drying powder, and compare the difference of antiviral activity between them in vitro and in vivo. Method： In vitro experiment, the 3-（ 4, 5-Dimethylthiazol-2-yl ）-2, 5-diphenyltetrazolium bromide （ MTT ） colorimetric method was applied to determine the 50% inhibitory concentration （IC50） against influenza virus FM1 of Jinqiao Reduqing granules and spray drying powder by different administration ways （drug was used directly on the virus or virus-infected cells）. Therapy indexes （T!） of them were also calculated. The mice were randomly divided into control group, Ribavirin （ 0.07 g·kg^-1 ）, Jinqiao Reduqing granules （ 11.7 g·kg^-1 ）, Jinqiao Reduqing spray drying powder （5.85, 11.7, 23.4 g .kg i）. In vivo experiment, the mice were infected with influenza virus FM1 through nasal inhalation, the mice were intragastric administrated after infection virus. The life-extension rate, lung index of infected mice were observed to investigate the protective function of Jinqiao Reduqing spray drying powder and Jinqiao Reduqing granules against influenza virus. Result： For two different administration ways, the ICs0 of Jinqiao Reduqing spray drying powder was 0.77, 0. 80 g·L^-1 , and the TI were 11.78, 11.34 respectively. The ICs0 of Jinqiao Reduqing granules were 3.70, 3.60 g·L^-1, and the TI were 6.37, 6.55 respectively. At the dose of 23.4 g·kg^-1 for Jinqiao Reduqing spray drying powder, its life-extension rate was 60. 81%, and the lung index inhibition rate was 13.18%. Compared with the virus control group, the difference were statistically significant （P 〈 0.05 ）. Conclusion： Jinqiao Reduqing spray drying powder and Jinqiao Reduqing granules have anti-influenza virus activity. But at the same dose, the anti-influenza virus FM1 function of Jinqiao Reduqing spray drying powder was more powerful than that of Jinqiao Reduqing granules in vitro.

关 键 词：金翘热毒清颗粒 金翘热毒清喷雾干燥粉 流感病毒 抗病毒作用 

分 类 号：R285.5[医药卫生—中药学]