前列腺素E_2受体激动剂和雌激素对奶牛输卵管上皮细胞中转化生长因子β_3表达的影响  

Effect of PGE_2 Receptor Agonist Butaprost and Estrogen on Expression of TGFβ_3 in Bovine Oviduct Epithelial Cell

作　　者：王毛毛[1,2] 毛伟[1,2] 曹金山[1,2] 董至恒[1,2] 刘博[1,2] 

机构地区：[1]内蒙古农业大学兽医学院,呼和浩特010018 [2]农业部动物疾病临床诊疗技术重点实验室,呼和浩特010018

出　　处：《中国畜牧兽医》2015年第6期1333-1339,共7页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金(31160490);新世纪优秀人才计划支持项目(NCET-11-109)

摘　　要：本试验旨在观察前列腺素E2受体激动剂(布他前列腺素(butaprost))与雌激素对奶牛输卵管上皮细胞中转化生长因子β3(TGFβ3)表达的影响,阐明butaprost和雌激素对奶牛输卵管上皮细胞TGFβ3有无协同调控作用。采用胰酶消化法及机械法分离培养奶牛输卵管上皮细胞,分别将butaprost和雌激素作用于体外培养的奶牛输卵管上皮细胞,采用实时荧光定量PCR技术检测butaprost和雌激素对奶牛输卵管上皮细胞中TGFβ3mRNA表达的影响。结果显示,与0h作用组相比,雌激素作用16、24和48h时对奶牛输卵管上皮细胞TGFβ3的表达量均极显著升高(P<0.01),4h的表达量极显著降低(P<0.01);且受体激动剂butaprost和雌激素有协同调控TGFβ3的效应;加入吲哚美辛后能有效抑制内源性前列腺素对TGFβ3表达的作用。结果表明,butaprost和雌激素可调控奶牛输卵管上皮细胞TGFβ3mRNA的表达。This study was aimed to observe effect of the prostaglandin receptor agonists butaprost and estrogen on TGFβ3 mRNA expression and illustrate whether butaprost and estrogen had coor- dinated regulation on bovine oviduct epithelial cells （BOECs） transforming growth factor β3 （TGFβ3） or not. BOECs were isolated and cultured by mechanical and pancreatic enzyme digestion method;butaprost and estrogen acted on BOECs, respectively;mRNA expression of TGFβ3 was detected by Real-time PCR in BOECs. The results showed that after the action of estrogen, the expression of TGF133 at 16,24 and 48 h compared with 0 h were extremely significantly increased （P〈0.01）, TGFβ3 mRNA expression reached minimal at 4 h （P〈0.01）;The receptor agonist butaprost and estrogen had coordinated regulation on TGFβ3. Adding indomethacin could inhibit the expression of endogenous prostaglandin for TGFβ3. The results showed that butaprost and es- trogen could regulate the expression of TGFβ3 mRNA in BOECs.

关 键 词：布他前列腺素(butaprost) 雌激素 奶牛输卵管上皮细胞 转化生长因子β3(TGFβ3) 

分 类 号：S823[农业科学—畜牧学]