圈养老年大熊猫肠道菌群16S rDNA克隆文库的建立  

Construction of the 16S rDNA Clone Library of Intestinal Flora of the Captive Elderly Giant Panda

作　　者：王晓艳[1,3] 袁听[1,3] 廖虹 罗永久[3] 何廷美[4] 王承东[4] 刘小敏[4] 吴虹林[4] 彭广能[3] 

机构地区：[1]重庆三峡职业学院动物科技系,重庆404100 [2]雅安市城乡规划建设和住房保障局,雅安625014 [3]四川农业大学动物医学院,动物疫病与人类健康四川省重点实验室,雅安625014 [4]中国林业科学研究院大熊猫保护研究中心,四川雅安碧峰峡基地,雅安625007

出　　处：《中国畜牧兽医》2015年第6期1402-1408,共7页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然基金面上项目(31272620);大熊猫国际合作资金项目(SD0632);教育部“长江学者和创新团队发展规划”创新团队项目(IRT0848)

摘　　要：为了研究老年大熊猫肠道菌群的构成,本试验对3只圈养老年大熊猫粪便细菌构建16SrDNA克隆文库,采用限制性内切酶HinfⅠ、MspⅠ对其进行限制性片段长度多态性(restriction fragment length polymorphism,RFLP)及测序分析。结果显示,老年大熊猫肠道细菌主要由变形菌门和厚壁菌门组成;变形菌门中又以大肠埃希氏菌属为主,其次为假单胞菌属、志贺氏菌属、气单胞菌属;而厚壁菌门中以链球菌属为主,其次为魏斯氏菌属、梭菌属;此外还发现一定比例的未培养细菌。本试验第一次建立了较丰富的老年大熊猫肠道菌群的克隆文库,为分析比较各年龄层大熊猫的肠道菌群结构的异同提供了参照,也为合理饲喂老年大熊猫,保障老年大熊猫的健康提供重要信息。In order to comparatively analyze the differences and similarities of intestinal flora from all ages of giant pandas,we built 16S rDNA clone library of three captive elderly giant pandas＇fe- cal bacteria and made restriction fragment length polymorphism （RFLP） and sequencing analysis by using restriction enzymes Hinf I and Msp I . The results showed that the intestinal flora of elderly giant panda were mainly attributable to two bacteria：Proteobacteria and Firmicutes. Be- tween them,the main in Proteobacteria of intestinal flora of elderly giant panda were Escherichia coli, followed by Pseudornonas sp. , Shigella and Aerornonas;The main in Firmicutes were Strep-tococcus, followed by Weissella ,Clostridium^Of which there were a certain proportion of bacteria were uncultured bacteria. The article firstly established a rich clone library of intestinal flora from elderly giant pandas, which provided a reference to comparatively analyze the similarities and differences in structure of intestinal flora from all ages of giant pandas, as well as provided the im- portant information about reasonable feeding elderly giant pandas and protecting health of elderly giant pandas.

关 键 词：圈养 老年大熊猫 肠道菌群 16SrDNA克隆文库 

分 类 号：S858.93[农业科学—临床兽医学]