中国美利奴羊OLA-DQB1基因exon 2遗传多态性与布鲁氏菌病易感性的关联分析  

The Correlation Analysis between Chinese Merino Sheep OLA-DQB1 Gene Exon 2 Genetic Polymorphism and Brucellosis Susceptibility

作　　者：王文文[1] 许万云[1] 胡梦薇[1] 李建华[1] 刘朋涛[1] 高剑峰[1] 

机构地区：[1]石河子大学生命科学学院,石河子832003

出　　处：《中国畜牧兽医》2015年第6期1495-1503,共9页China Animal Husbandry & Veterinary Medicine

基　　金：国家重点基础研究发展计划(973计划)(2010CB530200)

摘　　要：本试验采用PCR-SSCP方法对148只布鲁氏菌阴性和60只布鲁氏菌阳性中国美利奴羊白细胞表面抗原DQB1(OLA-DQB1)基因exon 2单核苷酸多态性(SNPs)进行了检测,之后挑选不同等位基因进行PCR产物测序,旨在确定该基因的多态性位点,并对每个SNP位点的等位基因频率、基因型频率进行统计分析,从而分析其多态性与布鲁氏菌病易感性的相关性。测序结果表明,在270bp的序列内共检测到43个SNPs,其中G196A位点的等位基因频率在病例组和对照组中的分布存在极显著差异(P<0.01),其基因型频率存在显著差异(P<0.05);C211T位点的等位基因频率在病例组和对照组中存在显著差异(P<0.05)。由此表明,OLA-DQB1基因exon 2多态性与中国美利奴羊布鲁氏菌病易感性呈显著相关。The single nucleotide polymorphisms （SNPs） of ovine lymphocyte antigen DQB1 （OLA-DQB1） gene exon 2 was amplified by PCR-SSCP method from 148 healthy and 60 infected with Brucella Chinese Merino sheep and then PCR products of different alleles were sequenced to determine the polymorphism loci of the gene. The differences in gene frequency and genotype fre- quency of each SNP loci were analyzed statistically to analyze its correlation with brucellosis sus- ceptibility. The sequencing result showed that 43 SNPs were detected in 270 bp DNA sequence, the gene frequencies of G196A allele had extremely significant difference in case and control sam- ples （P〈0.01） ,and its genotype frequencies presented significant difference （P〈0.05）. Similar- ly,C211T allele was significantly different in case and control samples （P〈0.05）. The results showed that the polymorphism of OLA-DQB 1 gene exon 2 might be a significant association gene with brucellosis susceptibility.

关 键 词：PCR-SSCP 中国美利奴羊 OLA-DQB1基因 单核苷酸多态性(SNPs) 布鲁氏菌病易感性 

分 类 号：S813.3[农业科学—畜牧学]