成都某种禽场鸡胚沙门氏菌的检测  

Detection of Salmonellain Chicken Embryos in a Breeding Poultry Field of Chengdu

作　　者：张丽芳[1] 肖桥[1] 罗薇[1] 

机构地区：[1]西南民族大学生命科学与技术学院,成都610041

出　　处：《中国畜牧兽医》2015年第6期1571-1579,共9页China Animal Husbandry & Veterinary Medicine

基　　金：四川省科技厅应用基础研究"丙型副伤寒沙门氏菌感染动物模型的建立及其致病机理的研究"(2013JY0045)

摘　　要：对四川省成都市某种禽场的156个死胚进行沙门氏菌的分离鉴定及药物敏感性检测。本试验采用沙门氏菌显色培养基、肠杆菌科生化鉴定管、三糖铁试验、沙门氏菌多价血清和16SrRNA PCR鉴定5种方法对疑似菌株进行鉴定,并用6种毒力岛基因将分离的沙门氏菌进行PCR鉴定。结果显示,沙门氏菌的分离率为15.4%(24/156),其中伤寒沙门氏菌占58.3%(14/24);fimY、invA和mgtC毒力基因的检测率均为100%;本试验分离菌对大部分沙门氏菌临床药物表现出明显的耐药性。In a poultry farm of Chengdu city, Sichuan province, isolation, identification and drug sensitivity test of Salmonella were conducted from 156 dead embryos. In this experiment,we used Salmonella chromogenic medium, biochemical identification of enterobacteriaceae, trisaccharide iron experiment,Salmonella polyvalent serum and 16S rRNA PCR to identify suspected strains, PCR identification of isolated Salmonella were conducted with 6 kinds of virulence genes. The re- sults concluded that Salmonella separation rate was 15.4 % （24/156）, including Salmonella typhi accounted for 58.3% （14/24）; Detection rates of fimY, invA and mgtC virulence genes were all 100% ;The isolated Salmonella were resistant to most clinical drugs to Salmonella.

关 键 词：死胚 分离 鉴定 毒力基因 药物敏感性 

分 类 号：S852.61[农业科学—基础兽医学]