Aggregation-induced emission （ALE） dye loaded polymer nanoparticles for gene silencing in pancreatic cancer and their in vitro and in vivo biocompatibility evaluation  

作　　者：Rui Hu Chengbin Yang Yucheng Wang Guimiao Lin Wei Qin Qingling Ouyang Wing-Cheung Law Quoc Toan Nguyen Ho Sup Yoon Xiaomei Wang Ken-Tye Yong Ben Zhong Tang 

机构地区：[1]School of Electrical and Electronic Engineering, Nanyang Technological University, Singapore 639798, Singapore [2]The Engineering Lab of Synthetic Biology and the Key Lab of Biomedical Engineering, School of Medicine, Shenzhen University, Shenzhen 518060, China [3]Department of Chemistry, The Hang Kong University of Science and Technology, Clear Water Bay, Kowloon, Hang Kong, China [4]Department of Industrial and Systems Engineering, The Hang Kong Polytechnic University, Hung Ham, Kowloon, Hang Kong, China [5]Division of Structural Biology & Biochemistry, School of Biological Sciences, Nanyang Technological University, Singapore 539798, Singapore

出　　处：《Nano Research》2015年第5期1563-1576,共14页纳米研究（英文版）

基　　金：This work was supported by the National Natural Science Foundation of China （NSFC） （61107017, 81301318）, the Start-up grant （M4080141.040） from Nanyang Technological University, Tier 1 Academic Research Funds （M4010360.040 RG29/10） from Singapore Ministry of Education and partially from the Singapore Ministry of Education under a Tier 2 Research Grant MOE2010-T2-2-010 （4020020.040 ARC2/11） and the grant from the Shenzhen Basic Research Project （JC201005280391A）

摘　　要：We have developed aggregation-induced emission （AIE） dye loaded polymer nanoparticles with deep-red emission for siRNA delivery to pancreatic cancer cells. Two US Food and Drug Administration （FDA） approved surfactant polymers, Pluronics F127 and PEGylated phospholipid, were used to prepare the dye-loaded nanoparticle formulations and they can be used as nanovectors for gene silencing of mutant K-ras in pancreatic cancer cells. The successful transfection of siRNA by the developed nanovectors was confirmed by the fluorescent imaging and quantified through flow cytometry. Quantitative real time polymerase chain reaction （PCR） indicates that the expression of the mutant K-ras oncogene from the MiaPaCa-2 pancreatic cancer cells has been successfully suppressed. More importantly, our in vivo toxicity study has revealed that both the nanoparticle formulations are highly biocompatible in BALC/c mice. Overall, our results suggest that the AIE dye-loaded polymer nanoparticle formulations developed here are suitable for gene delivery and have high potential applications in translational medicine research.

关 键 词：aggregation-induced emission polymer nanoparticles gene silencing pancreatic cancer 

分 类 号：Q522[生物学—生物化学] TQ174.75[化学工程—陶瓷工业]