复方夏枯草活性部位对离体大鼠胸主动脉的舒张作用及机制研究  

Vasodilation Effect and Underlying Mechanism of Active Fraction from Compound Prunella Vulgaris L. in Isolated Thoracic Aorta of Rats

作　　者：雀苏云 楼一层[1] 侯靖[1] 

机构地区：[1]武汉理工大学化学化工与生命科学学院,武汉430070

出　　处：《中国药师》2015年第6期884-887,共4页China Pharmacist

基　　金：国家自然科学基金项目(编号81173509)

摘　　要：目的：探讨复方夏枯草活性部位（AFCP）对大鼠离体胸主动脉的作用及其可能的机制。方法：采用离体大鼠胸主动脉张力实验,经生物信号采集与分析系统测定血管环张力的变化,观察AFCP的舒张血管作用。结果：AFCP（100-500μg·ml^-1）能显著降低苯肾上腺素（PE,1μmol·L^-1）引起的血管收缩,对内皮完整和去内皮血管均有（75%±8%）舒张作用。AFCP的舒血管作用不受一氧化氮合酶抑制药L-NNA、岛苷酸环化酶抑制药MB、钾通道阻滞药TEA和格列苯脲的影响。在无钙K-H液中AFCP（300μg·ml^-1）可使Ca Cl2的量效曲线下移且使PE的最大收缩幅度降低。结论：AFCP够浓度依赖性舒张大鼠胸主动脉,其舒张血管作用无内皮依赖性。其作用机制可能与抑制细胞内钙离子释放和细胞外钙离子内流有关,与NO途径、前列环素生成和钙激活的钾通道无关。Objective： To investigate the vasorelaxant effect of active fraction from compound Prunella vulgaris L. （AFCP） on the isolated thoracic aorta of rats and underlying mechanism. Methods： The study was performed with the tension experiment of the isolate rat thoracic aorta. The changes of vascular ring tension were measured by biological signal acquisition and analysis system, and the vasodilator effect of AFCP was observed. Results： AFCP（ 100-500μg ·ml^-1 ）could induce significant relaxation in aorta rings pre - contracted by phenylephrine （PEμmol ·ml^-1）, and the relaxation effect was significant（75% ±8% ） in endothelium-intact aortic and endothelium- denuded aortic. The vasodilatation effect of AFCP was not significantly affected by nitric oxide synthase （NOS） inhibitor NG-nitro-L-argi- nine （L-NNA）, guanylate cyclase inhibitor MB, potassium channel blocker TEA and glibenclamide. In Ca^2＋ -free bath solutions, AFCP （300 μg ml^-1 ） could shift downward dose-response curve of CaCl2 and significantly reduce the maximum contraction amplitude of PE. Conclusion： AFCP can relax rat aorta ringsrin a dose-dependent manner, which is endothelium-independent. The mechanism may be related to the inhibition of intracellular calcium release and extracellular calcium flow, and has nothing to do with NO pathway, prostacyclin generation and calcium-activated potassium channels.

关 键 词：复方夏枯草提取物 胸主动脉 血管舒张作用 

分 类 号：R285.5[医药卫生—中药学]