纤花线纹香茶菜中3种水溶性黄酮类成分含量动态变化研究  

Study on Content Dynamic Changes of Three Water-soluble Flavonoids in Isodon Lophanthoides

作　　者：龚明[1] 黄杨志 唐海明[3] 贺莲[4] 

机构地区：[1]怀化医学高等专科学校,湖南怀化418000 [2]东莞市谢岗医院 [3]广州中医药大学 [4]中山大学新华学院

出　　处：《中国药师》2015年第6期949-951,969,共4页China Pharmacist

基　　金：国家科技支撑计划项目(编号:2012BAI29B09)

摘　　要：目的：建立同时测定纤花线纹香茶菜中新西兰牡荆苷2、异夏佛塔苷、夏佛塔苷含量的方法,并分析其含量动态变化。方法：采用Phenomenex luna C18（250 mm×4.6 mm,5μm）色谱柱,流动相为甲醇-0.5%甲酸水溶液,梯度洗脱,流速为0.8ml·min^-1,检测波长为330 nm,柱温为室温（25℃）,进样量：10μl。结果：新西兰牡荆苷2、异夏佛塔苷、夏佛塔苷线性范围分别为0.130-3.110μg（r=0.999 8）、0.180-4.540μg（r=0.999 9）、0.08-1.97μg（r=0.999 9）;平均回收率分别为96.8%、99.2%、97.1%,RSD分别为1.9%、1.6%、1.6%（n=6）。上述3种水溶性黄酮成分从幼苗期开始显著累积,异夏佛塔苷和夏佛塔苷在3月和8月达到最高值;新西兰牡荆苷2的含量在1~4月趋于稳定,5月后开始逐渐累积,8月达最高值,之后开始呈逐渐下降的趋势。结论：以水溶性黄酮成分含量为指标,纤花线纹香茶菜的最佳采收期为3月和8月,且以8月份采收的药材质量最佳。Objective： To establish an HPLC method for the simultaneous determination of vicenin H , isoschaftoside and schaftoside in Isodon lophanthoides, and analyze their content dynamic changes. Methods： The HPLC system consisted of a Phenomenex luna Cls （250 mm × 4.6 mm, 5 Ixm）column and a solution system of methanol and 0.5% formic acid with gradient elution, the flow rate was 0.8 ml · min^ -1 and the detection wavelength was 334 nm at the column temperature of 25℃and the injection volume was 10 μl. Results ： The linear range of vicenin H , isoschaftoside and schaftoside was 0. 130-3.110 μg （ r = 0.999 8 ）, 0. 180-4. 540 μg （ r = 0.999 9） and 0.080-1.970 μg（r =0. 9999）, respectively. The average recovery was 96.8% （RSD = 1.9% ）, 99.2% （RSD = 1.6% ） and 97.1% （ RSD = 1.6% ） （ n = 6）, respectively. The above three kinds of water-soluble flavonoids from seedling stage to significant accumulation, isoschaftoside and schaftoside reached the highest value in March and August, the content of Vitexin H in January and April tends to be stable, in May began to increase gradually, reached the maximum value in August, then began to decrease. Conclusion： With the contents of water-soluble flavonoids as the indicators, the best harvest time of Isodon lophanthoides is March and August, and the quality of the medicinal materials harvested in August is the best.

关 键 词：高效液相色谱法 纤花线纹香茶菜 新西兰牡荆苷2 异夏佛塔苷 夏佛塔苷 

分 类 号：R927.2[医药卫生—药学]