HPLC法测定复方二氧化钛乳膏中水杨酸苯酯含量  

Determination of Phenyl Salicylate in Compound Titanium Dioxide Cream by HPLC

作　　者：宋立娟[1] 徐风华[1] 孙华燕[1] 李婕[1] 马晓娟[1] 

机构地区：[1]解放军总医院制剂室,北京100853

出　　处：《中国药师》2015年第6期1036-1038,共3页China Pharmacist

基　　金：军队医疗机构制剂标准提高科研专项课题(编号:13ZJZ05)

摘　　要：目的：建立HPLC法测定复方二氧化钛乳膏中水杨酸苯酯的含量。方法：采用Zorbax SB C18色谱柱（250 mm×4.6 mm,5μm）,以甲醇-水（78∶22）为流动相,流速为1.0 ml·min^-1,检测波长为308 nm,柱温为25℃。结果：水杨酸苯酯在59.460~198.200μg·ml-1浓度范围内呈良好线性关系（r=0.999 5）;平均回收率为101.84%,（RSD=0.64%,n=9）;3批复方二氧化钛乳膏中水杨酸苯酯的含量分别为107.7%、107.5%、109.8%。结论：该方法简便快捷,准确、灵敏,专属性强,适用于复方二氧化钛乳膏中水杨酸苯酯的含量测定。Objective ： To establish a method for the determination of phenyl salicylate in compound titanium dioxide cream. Methods： The HPLC assay was carried out on an Agilent Zorbax SB C18 （250 mm ×4.6 mm, 5 μm） column with methanol - water （78： 22） as the mobile phase. The sample was detected at 308 nm with a UV detector. The column temperature was set at 25 ℃ and the flow rate was 1.0 ml·min^ -1. Results ： Phenyl salicylate could isolate from the other materials by HPLC. The linear range of phenyl salicylate was 59. 460-198. 200 μg · ml^-1 （ r = 0. 999 5 ）. The recovery was 101.84% with RSD of 0.64% （ n = 9）. The content of phenyl salicylate in 3 batches of compound titanium dioxide cream was 107.7%, 107.5% and 109.8%, respectively. Conclusion： The method is simple, rapid, exclusive, accurate and sensitive, which is suitable for the determination of phenyl salicylate in compound titanium dioxide cream.

关 键 词：复方二氧化钛乳膏 水杨酸苯酯 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]