卷烟烟气总粒相物诱导A549细胞的氧化应激研究  

Oxidative Stress in A549 Cells Induced by Total Particulate Matter in Cigarette Smoke

作　　者：张世敏[1] 李翔[1] 谢复炜[1] 谢剑平[1] 

机构地区：[1]中国烟草总公司郑州烟草研究院

出　　处：《烟草科技》2015年第6期52-56,共5页Tobacco Science & Technology

基　　金：国家科技支撑计划项目"食品中热反应伴生危害物毒理学检测及风险评估关键技术研究"(2012BAK01B03);中国烟草总公司郑州烟草研究院科技项目"基于MRTP健康风险评估框架的体外毒理学测试方法研究"(322013CZ0600)

摘　　要：为研究卷烟烟气总粒相物(TPM)对人肺腺癌细胞(A549)的氧化损伤,捕集3R4F参比卷烟主流烟气的TPM,对A549细胞进行染毒,采用中性红细胞毒性法测试TPM与A549细胞存活率的剂量-效应关系,检测了细胞内谷胱甘肽(GSH)和细胞外超氧化物歧化酶(EC-SOD)两种氧化应激指标。结果表明:1TPM与A549细胞存活率之间存在良好的剂量-效应关系。2细胞发生氧化应激时,细胞内还原态谷胱甘肽和氧化态谷胱甘肽比值(GSH/GSSG)相对于阴性对照组有显著降低,且不随染毒时间的增加而变化;EC-SOD在染毒4 h后没有显著增加,但24 h后有显著增加。In order to evaluate the oxidative stress of human lung adenocarcinoma cells （A549 cells） induced by total particulate matter （TPM） in cigarette smoke, A549 cells were exposed to the TPM of mainstream smoke of 3R4F reference cigarette. The dose-response relationship between TPM dose and the viability of A549 cells was tested by the neutral red assay, and two indicators of oxidative stress, intracellular glutathione （GSH） and extracellular superoxide dismutase （EC-SOD）, were determined. The results indicated that： 1）A good dose-response relationship existed between TPM dose and the viability of A549 cells. 2） When oxidative stress occurred, the rate of reduced and oxidized state glutathiones （GSH/ GSSG） in cells significantly decreased comparing with negative control, it did not change with the increase of exposure duration. EC-SOD did not increase significantly in 4 hours, while it increased significantly after 24-hour exposure. The experimental results provide a reference for the research of cells＇ oxidative stress induced by cigarette smoke.

关 键 词：卷烟烟气 总粒相物(TPM) 人肺腺癌细胞(A549) 谷胱甘肽(GSH) 细胞外超氧化物歧化酶(EC—SOD) 氧化应激 

分 类 号：TS416[农业科学—烟草工业]