HPLC法测定蛋黄油中胆固醇含量  

Determination of Cholesterol in Egg Yolk Oil by HPLC

作　　者：王小妹[1] 刘春芳[1] 杨阳[1] 

机构地区：[1]中药提取分离过程现代化国家工程研究中心/广州白云山汉方现代药业有限公司,广东广州510240

出　　处：《今日药学》2015年第5期328-330,337,共4页Pharmacy Today

基　　金：广州市药用脂质重点实验室项目(穗科信字[2013]163号)

摘　　要：目的建立高效液相色谱法测定蛋黄油中胆固醇的含量。方法样品无须皂化处理,直接用二氯甲烷-甲醇（1∶2）溶解,经反相C18色谱柱（150 mm×3.9 mm,5μm）分离后测定,以甲醇为流动相A,以甲醇-异丙醇-正已烷（10∶70∶20）为流动相B;梯度洗脱;检测波长为205 nm;流速为1.0 m L/min;柱温为35℃。结果本方法测定胆固醇线性范围为0.097 23～1.94463 mg/m L,线性方程y=3 869.446x＋15.519,相关系数r=0.999 9,线性关系良好;加样回收率为96.98%～100.4%。结论该方法灵敏、快速、准确,适用于测定蛋黄油中胆固醇的含量。OBJECTIVE To establish a method for determination of Cholesterol in egg yolk oil by HPLC. METHODS The sample was dissolved in Dichloromethane-Methanol（ 1∶ 2） without saponification. The chromatographic separation was carried out on a C18（ Waters C18： 150 mm × 3. 9 mm,5 μm） column using UV detector at 205 nm. Methanol and Methanol-Isopropyl alcohol-n-hexane（ 10∶ 70 ∶ 20） were used as mobile phase by gradient elution. The flow rate was 1. 0 m L/min,column temperature was 35 ℃.RESULTS The linear range of Cholesterol was 0. 097 23- 1. 944 63 mg / m L,Linear equation was y = 3 869. 446 x ＋ 15. 519（ r = 0.999 9）. The average recovery range was 96. 98%- 100. 4%. CONCLUSION The method is simple,quick and accurate. It can be used for determination of Cholesterol in egg yolk oil.

关 键 词：胆固醇 蛋黄油 高效液相色谱法 含量测定 

分 类 号：R285[医药卫生—中药学]