鱼卵孵化酶在黄颡鱼鱼卵中的免疫组织化学定位和分布  

Immunohistochemistry Localization of Hatching Enzyme in the Developing Embryos of Pelteobagrus fulvidraco

作　　者：李浩然[1] 蒋石容 欧仁建 郭微微[1] 杨先乐[1] 胡鲲[1] 

机构地区：[1]上海海洋大学,国家水生动物病原库,上海201306 [2]湖南省华容县水产局,湖南岳阳414200 [3]四川省成都市龙泉驿区水务局,成都610100

出　　处：《四川动物》2015年第3期352-356,共5页Sichuan Journal of Zoology

基　　金：科技部863计划项目“新型环境友好型渔药的研制与渔药安全性评价”(2011AA10A216);农业公益性行业专项“渔药使用风险评估及其控制技术研究与示范”(201203085);国家自然科学基金项目“鱼类GABA受体鱼渔药安全性评价的研究”(31172430);上海市科技兴农重点攻关项目(沪农科攻字(2011)第4~8号);上海高校知识服务平台;国家科技基础条件平台建设运行项目

摘　　要：孵化酶存在于各种水生动物的胚胎中,会影响水生生物胚胎发育,是造成鱼类人工繁殖过程中早脱膜现象的重要因素之一。本研究以黄颡鱼鱼卵为研究对象,采用免疫组织化学方法对不同孵化时间点的孵化酶进行定位分布研究,探讨孵化酶在黄颡鱼鱼卵中的时空表达规律。结果表明:在黄颡鱼鱼卵中,孵化酶主要分布在鱼卵外胚层及卵膜内层上;在鱼卵孵化的各个时期均有孵化酶存在,孵化酶在各时间点的相对表达量没有明显差异。研究结果为阐释孵化酶在鱼卵孵化过程中的作用奠定了基础。Hatching enzyme was a major factor to cause the early stripping during the fish artificial propagation, which ex- isted in the embryos of a variety of aquatic species and affected embryo growth. In this study, the embryo of Peheobagrus fulvidraco was chosen to measure the localization of hatching enzyme during different incubation periods using immunohisto- chemical method, and to explore the temporal and spatial distribution expression of hatching enzyme in P. fulvidraco embry- os. The results revealed that hatching enzyme mostly distributed in germ layer and inner layer of the outer embryo mem- brane. In addition, hatching enzyme existed in each embryo period, and non-significant expression was found at each time point. The information presented in this study was vital for the research on the hatching enzyme mechanism of action of inter- pretation in the process of P. fulvidraco embryo hatch.

关 键 词：孵化酶 黄颡鱼 鱼卵 免疫组织化学 定位 

分 类 号：S917.4[农业科学—水产科学]