重组人促卵泡激素剂量对食蟹猴卵泡发育和排卵同期化的影响  

Effects of rh FSH on Ovarian Follicular Development and Ovulation Synchronization in Macaca fascicularis

作　　者：张秀娟[1] 陈金平[1] 张全军[2] 刘朝明[2] 刘晓明[1] 赖良学[2] 张守全[3] 

机构地区：[1]广东省昆虫研究所,广州510260 [2]中国科学院广州生物医药与健康研究所,广州510530 [3]华南农业大学动物科学学院,广州510642

出　　处：《四川动物》2015年第3期407-410,共4页Sichuan Journal of Zoology

基　　金：广州市重大科技专项项目(No.2010U1-E00811);国家重点基础研究发展规划(973计划)项目(2010CB945001;2011CB944202)

摘　　要：目的优化食蟹猴胚胎移植同步受体的处理技术。方法选择5～9岁月经周期正常的成年雌性食蟹猴37只,于出现月经血的第2～3天,每日肌注重组人促卵泡激素（rh FSH）,按超排剂量和方式分2个实验组,超排供体组、超排受体组,另有一组自然受体组（对照组）。结果和结论 2个实验组超排后卵巢反应全部良好,超排供体组和超排受体组超排后卵巢的获卵总数（15.18±6.51 VS.5.67±3.79）和平均排卵数（6.77±3.61 VS.1.00±0.00）差异具有统计学意义（P〈0.05）,但超排受体组和超排供体组的雌二醇（E2）水平浓度差异无统计学意义（P〉0.05）,变化模式一致,且超排受体组与超排供体组排卵同期化的准确率可达83.33%,而对照组仅为42.85%,两者差异具有统计学意义（P〈0.05）。Objective To optimize effective treatment technology of embryo recipient from Macaca fascicularis. Method A total of 37 adult female M. fascicularis （5 -9 years old） were housed in seperated cages and the vaginal bleeding was mo- nitored daily to detect the onset of menses. Monkeys with menstrual bleeding in 3 days after recombinant human follicular stimulating hormone （rhFSH） injection were classified into three groups： stimulation donors （SD, n = 13 ）, stimulation re- cipients （SR, n = 6 ） and nature recipients （NR, n = 21 ）. Result and Conclusion Uhrasonograms results showed that the dual-side of ovaries in SD and SR groups were good on day 8 after rhFSH stimulation. Statistical analysis indicated the number of recovered oocytes and ovulated eggs in SR were lower than those in SD （5.67 ± 3.79 versus 15.18 ±6.51, 1.00 ± 0.00 versus 6.77 ± 3.61, respectively, P 〈 0.05 ）, however, the values and profiles of serum E2 concentrations were significantly similar （P 〉 0.05 ）. Meanwhile, the accuracy rate of ovulation synchronization was 83.33% between SR and SD, but only 42.85% between NR and SD. There were significant difference between experiment and control group （P 〈0.05 ）.

关 键 词：食蟹猴 卵泡发育 排卵同期化 

分 类 号：Q954.4[生物学—动物学]