棉花LTPG基因家族的蛋白结构与基因表达分析  

Protein Structure and Gene Expression Pattern Analysis of Gossypium raimondii GPI-anchor Lipid Transfer LTPG Family

作　　者：邓婷[1] 王劲[2] 王俊[1] 周玉梅[1] 左开井[1] 

机构地区：[1]上海交通大学农业与生物学院,上海200240 [2]中国农科院生物技术研究所,北京100081

出　　处：《中国农业科技导报》2015年第2期49-57,共9页Journal of Agricultural Science and Technology

基　　金：国家转基因生物新品种培育重大专项(2013ZX08005-003);国家自然基金项目(31071458)资助

摘　　要：大量研究表明脂类化合物参与了棉花纤维的起始与伸长发育,GPI锚定的脂质转运蛋白(LTPG)是脂类细胞器之间运输的候选蛋白,对雷蒙德棉(D组)的LTPG家族的蛋白质结构与基因表达进行了分析。结果发现:LTPG基因家族可以分为8个亚类;不同亚类的蛋白的保守半胱氨酸残基位置/分布等具有显著特征;LTPG基因的启动子具有MYB等多种转录因子结合元件以及多种激素应答元件;LTPG基因家族在纤维发育过程中存在着3种不同的表达模式。上述结果表明LTPG基因家族可能参与棉花纤维起始与伸长发育,其功能值得深入分析。A lot of research indicates that lipids are involved in cotton fiber initiation and elongation. LTPG （ GPI- anchored lipid transfer protein） is involved in lipid transportation during cell enlargement, so the protein structure and gene expression pattern of LTPG gene family in Gossypium raimondii were analyzed. The results showed that LTPGs were classified into 8 subgroups, and the position/distribution of each subgroup protein had its own typical molecular weight and conserved cysteine motif. Promoter elements analysis indicated that LTPGs contained the conservative binding sites for MYB transcription factors and hormone response elements. RNA-seq data revealed that three expression patterns of LTPGs existed during fiber development, suggesting their roles in fiber initiation and elongation.

关 键 词：纤维伸长发育 脂质 脂质转运蛋白(LTPG) 表达分析 

分 类 号：Q78[生物学—分子生物学]