过敏性皮炎患者IL-4和IL-13以及EBV抗体的检测及临床意义  

Detection and Significance of IL-4 and IL-13 and EBV Antibody in Patient with Atopic Dermatitis

作　　者：刘光震[1] 颜廷斐[2] 李慧[2] 

机构地区：[1]青岛大学附属医院放射科,山东青岛266003 [2]青岛大学附属医院检验科,山东青岛266003

出　　处：《医药前沿》2015年第12期68-70,共3页Journal of Frontiers of Medicine

摘　　要：目的：探讨人血清IL-4和IL-13水平以及EBV感染在过敏性皮炎发生中的作用，分析过敏性皮炎患者EBV感染与患者血清IL-4和IL-13水平的相互关系。方法：采用酶联免疫吸附试验（ELISA）检测30例过敏性皮炎患者和40例健康对照组血清IL-4和IL-13水平以及血清EB病毒早期抗原抗体EA—IgM和EA—Igh以及衣壳抗原抗体VCA—IgM，VCA—IgA和VCA-IgG含量，并进行相关性研究。结果：过敏性皮炎患者血清IL-4（9．40±3．35pg／ml），IL-13（4．62±1．00pg／ml），EA-IgM（4．38±3．39U／ml），EA—IgA（1．46±0．36U／m1）和VCA—IgM（8．49±2．71U／m1）水平明显高于正常对照组（3．88±1．73pg／ml，2．87±1．08pg／ml，2．68±1．12U／ml，1．29±0．29U／m1和5．61±1．95U／ml：P〈0．001，P〈0．001，P〈0．05，P〈0．05和P〈0．001）。过敏性皮炎患者血清VCA—IgA和VCA—IgG水平分别为5．78±3．26U／ml和109．86±74．43U／ml，对照组血清VCA—IgA和VCA—IgG水平分别为8．01±19．30U／ml和121．48±122．11U／ml，统计学分析表明两组间无显著性差异（P均〉0．05）。患者血清IL-4和IL-13水平与EBV抗体EA—IgM，EA—IgA，VCA—IgM，VCA—IgA以及VCA—IgG水平无明显相关性（P均〉0．05）。结论：过敏性皮炎的发生发展可能与血清IL-4和IL-13水平升高有关；过敏性皮炎患者存在EBV的活动性感染；EBV感染可能不是导致过敏性皮炎患者血清IL-4和IL-13水平升高的影响因素。Objective To investigate the association of IL-4 and IL-13 level in serum and EBV infection in the development of atopic dermatitis. To analysis the correlations between EBV infection and IL-4 and IL-13 level in serum in patients with atopic dermatitis.Methods The serum levels of IL-4 and IL-13 and the serum contents of antibodies against F.BV early antigen EA-IgM and EA-IgA and viral capsid antigen VCA-IgM,VCA-IgA and VCA-IgG of 30 atopic dermatitis patients and 40 healthy controls were detected using ELISA. Results The serum levels of IL-4, IL-13, EA-IgM, EA-IgA and VCA-IgM in the atopic dermatitis group were significantly higher than that in the control group（P〈0.001,P 〈0.001,P〈0.05,P〈0.05 and P〈0,001）. The EBV VCA-IgA and VCA-IgG levels of the atopic dermatitis group and control group in serum were 5.78±3.26U/ml and 109.83±74.43U/ml, 8.01±19.30U/ml and 121.48±122.11U/ml respectively, there was not significantly difference between two groups.There was no association between IL-4, IL-13 and EBV EA- IgM, EA-IgA, VCA-IgM,VCA-IgA and VCA-IgG in patients with atopic dermatitis （P〉0.05）. Conclusion The occurrence and development of atopic dermatitis was probably concerned with the increased expression of IL-4 and IL-13 in serum. The EBV active infection was exist in atopic dermatitis patients.The EBV infection may not be influencing factor what result in the increased expression of IL-4 and IL-13 in serum in atopic dermatitis patients.

关 键 词：过敏性皮炎 白细胞介素4 白细胞介素13 EB病毒 抗体 

分 类 号：R446[医药卫生—诊断学]