辛基酚对5-羟色胺转运及受体通路的影响  

Influence of Octylphenol on 5-HT_(3b) and 5-HT transporter in human liver 7702 cells

作　　者：杨婕[1] 褚玥[1] 赖玉婷[1] 羿利华[1] 杨童旺 郭佑廷 黄少文[1] 柳春红[1] 

机构地区：[1]华南农业大学食品学院(广东省食品质量安全重点实验室),广东广州510642

出　　处：《中国现代医学杂志》2015年第14期11-16,共6页China Journal of Modern Medicine

基　　金：广东省自然科学基金(No:S2011010001045);广东省科技计划项目(No:2012B090600005);农业部农产品质量安全监管项目(No:GTFP2014011)

摘　　要：目的通过观察人肝7702细胞暴露于辛基酚(OP)后5-羟色胺(5-HT)转运体SERT、5-HT3b受体表达以及5-HT水平的变化,探讨OP对5-HT转运通路和受体结合通路的影响。方法实验分5个组,分别为OP高(5.0μg/L)、中(1.0μg/L)、低(0.2μg/L)剂量组、盐酸氟西汀组和空白对照组,体外细胞培养48 h,采用ELISA试剂盒检测细胞上清液中5-HT含量的变化,运用实时荧光定量PCR法检测转运体SERT及5-HT3b受体的表达。结果中、高剂量组细胞上清液中5-HT含量高于空白对照组(P<0.05),细胞中5-HT3b受体的表达降低(P<0.05);低、中、高剂量组细胞中SERT的表达均低于空白对照组(P<0.05);与盐酸氟西汀组检测结果一致。结论 OP可引起5-HT再摄取障碍,同时减少5-HT3b受体的表达,对机体5-HT代谢网络的转运通路及受体结合通路产生毒效应作用。[Objective] To explore the influence of Octylphenol （OP） on 5-hydroxytryptamine （5-HT） re- ceptor 5-HT3b and serotonin transporter （SERT） in human liver （HL） 7702 cells by means of detecting the level of 5-HT and observing expression levels of SERT and 5-HT3b in these cells. [Methods] Five groups were set for the study. Fluoxetine was for positive control, meanwhile other cultured cells were treated with different doses of OP [OP high （5 p.g/L）, middle （1 I^g/L）, low （0.2 p^g/L）] and the blank control group with in vitro cell culture for 48 h. The content of 5-HT in HL-7702 cells supernatant was detected by enzyme linked immunosorbent assay （ELISA）. The expressions of SERT mRNA and 5-HT3b mRNA in HL-7702 cells were detected by real-time fluorescent quantitative PCR. [ Results ] The content of 5-HT in 1 Ixg/L OP and 5 Ixg/L OP groups was significantly higher than that in the blank control group （P〈 0.01）, but the 5-HT3b levels were significantly decreased in the three OP groups （P 〈 0.05）. The expressions of SERT in the OP groups signifi- cantly decreased compared to the blank control group （P 〈 0.05）, the result was consistent with that of the flu- oxetine group. [ Conclusion] OP exposure causes the disorders of reuptake and binding with receptors of 5- HT through inhibiting SERT and 5-HT3b, so produces combined toxic effect in the transport pathway and re- ceptor binding pathway.

关 键 词：辛基酚 5-羟色胺 5-羟色胺转运体 5-羟色胺受体 人肝7702细胞 

分 类 号：R114[医药卫生—卫生毒理学]