抑制HO-1表达对GLU和AGEs刺激下THP-1细胞氧化应激的影响研究  

Study Effect of Inhibition of HO- 1 Expression on High Glucose and AGEs Stimulated THP- 1 Cells Oxidative Stress

作　　者：林明 朱旭新[2] 谢晓原[2] 林谭发[2] 

机构地区：[1]汕头市潮南区人民医院急诊,广东汕头515144 [2]汕头大学医学院第一附属医院内分泌科,广东汕头515041

出　　处：《黑龙江医学》2015年第5期465-467,共3页Heilongjiang Medical Journal

基　　金：汕头市科技局(汕府科[2012]113号)

摘　　要：目的探讨抑制血红素加氧酶1(HO-1)表达对高糖(GLU)和糖基化终产物(AGEs)刺激下人单核细胞株(THP-1)氧化应激的影响。方法将THP-1细胞分为三组培养,包括对照组、GLU+AGEs组(含15 mmol/L的GLU和100 g/m L的AGEs的培养液)、Zn PP(锌原卟啉)+GLU+AGEs组(先予20 mol/L Zn PP预处理30 min后再以15 mmol/L的GLU和100 g/m L的AGEs刺激),检测各组肿瘤坏死因子α(TNF-α)、丙二醛(MDA)和细胞活性氧(ROS)水平以及HO-1 mRNA和蛋白表达。结果 GLU+AGEs组ROS、MDA和TNF-α水平分别为(101.28±8.67)MFI、(3.17±0.58)nmol/m L和(40.28±10.67)pg/m L,明显高于对照组(P<0.05);Zn PP+GLU+AGEs组ROS、MDA和TNF-α水平分别为(122.47±9.11)MFI、(3.84±0.78)nmol/m L和(132.27±11.09)pg/m L,明显高于GLU+AGEs组和对照组(P<0.05);GLU+AGEs组和Zn PP+GLU+AGEs组HO-1 mRNA表达量明显高于对照组(P<0.05),其中Zn PP+GLU+AGEs组HO-1 mRNA表达量低于GLU+AGEs组(P<0.05);GLU+AGEs组和Zn PP+GLU+AGEs组HO-1蛋白表达量明显高于对照组(P<0.05),其中Zn PP+GLU+AGEs组HO-1蛋白表达量低于GLU+AGEs组(P<0.05)。结论高糖和AGEs可引起THP-1细胞氧化损伤和HO-1表达增高,而抑制HO-1表达可进一步加剧高糖和AGEs引起的细胞氧化损伤。Objective To investigate the inhibition heme oxygenase 1 （HO-1） expression on high glucose （GLU） and advanced gly-cation end products （AGEs） stimulated human monocytes （THP-1） oxidative stress effect.Methods THP-1 cells were divided into three groups, including control group, GLU＋AGEs group （medium containing 15 mmol/L GLU and 100 g/mL AGEs）, ZnPP＋GLU＋AGEs group （first,20 mol/L ZnPP 30min after pretreatment with 15mmol/L GLU and 100 g/mL AGEs stimulus）, detected each group of tumor necrosis factor-α（ TNF-α） , malondialdehyde （ MDA） and cellular reactive oxygen species （ ROS） level and expression of HO-1 mRNA and protein.Results GLU＋AGEs group of ROS, MDA and TNF-αlevels were （101.28 ±8.67） MFI, （3.17 ±0.58） nmol/mL and （40.28 ±10.67）pg/mL, significantly higher than the control group （P〈0.05）;ZnPP＋GLU＋AGEs group of ROS, MDA and TNF-αlevels were （122.47 ±9.11） MFI,（3.84 ±0.78）nmol/mL and （132.27 ±11.09）pg/mL, were significantly higher than that of GLU＋AGEs group and the control group （P〈0.05）;GLU＋AGEs group and ZnPP＋GLU＋AGEs group HO-1 mRNA expression was significantly higher than that of the control group （P〈0.05）, the HO-1 mRNA expression in ZnPP＋GLU＋AGEs group was lower than that of GLU＋AGEs group （ P〈0.05 ）; GLU＋AGEs group and ZnPP＋GLU＋AGEs group HO-1 protein expression was significantly higher than the control group （P〈0.05）, the ZnPP＋GLU＋AGEs group HO-1 protein expression was lower than that in GLU＋AGEs group （P 〈0.05）.Conclusion High glucose and AGEs can cause THP-1 cells oxidative damage and HO-1 expression increased, the inhibition of HO-1 expression may further exacerbated high glucose and AGEs caused cellular oxidative damage.

关 键 词：高糖 糖基化终产物 血红素加氧酶1 人单核细胞株 氧化应激 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]