多波长HPLC法同时测定心宁片中5种成分的含量  

Content Determination of 5 Components in Xinning Tablets by Multi-target with Multi-wavelength HPLC

作　　者：张熙洁[1] 吴江 姜晖[1] 刘晓红[1] 

机构地区：[1]唐山市工人医院,河北唐山063000 [2]解放军第255医院,河北唐山063000

出　　处：《中国药房》2015年第18期2535-2538,共4页China Pharmacy

基　　金：河北省自然科学基金石药集团医药联合研究基金资助项目(No.C2011105042)

摘　　要：目的：建立同时测定心宁片中丹参素、原儿茶醛、丹酚酸B、芍药苷和阿魏酸含量的方法。方法：采用多波长高效液相色谱法。色谱柱为Agilent Zorbax Eclipse XDB-C18,流动相为乙腈-甲醇-0.5%H3PO4溶液（梯度洗脱）,流速为1.0 ml/min,柱温为30℃,进样量为10μl,检测波长为280 nm（丹参素、原儿茶醛、丹酚酸B）、230 nm（芍药苷）、320 nm（阿魏酸）。结果：丹参素、原儿茶醛、丹酚酸B、芍药苷、阿魏酸质量浓度分别在66.25~1 060.00、5.55~88.86、187.20~2 995.20、23.71~379.39、0.20~3.12μg/ml范围内与各自峰面积呈良好的线性关系（r=0.999 9、0.999 9、0.999 7、0.999 9、0.999 7）;精密度、稳定性、重复性试验的RSD〈2%;平均加样回收率分别为98.85%、97.95%、99.18%、98.14%、97.16%,RSD分别为0.12%、0.19%、0.37%、0.25%、1.36%（n=6）。结论：该方法分离效果好、操作简便,可用于测定心宁片中5种成分的含量。OBJECTIVE：To establish a method for the content determination of tanshinol,protocatechuic aldehyde,salvianolic acid B,paeoniflorin and ferulic acid in Xinning tablets. METHODS：Multi-target with multi-wavelength HPLC was performed on the column of Agilent Zorbax Eclipse XDB-C18 with the mobile phase of acetonitrile-methanol-0.5% H3PO4 aqueous solution（gradient elution）at the flow rate of 1.0 ml/min. The column temperature was 30 ℃,volume was 10 μ l and the detection wavelength was 280 nm for tanshinol,protocatechuic aldehyde and salvianolic acid B,230 nm for paeoniflorin and 320 nm for ferulic acid.RESULTS：The linear range was 66.25-1060.00 μ g/ml for tanshinol（r=0.999 9）,5.55-88.86 μ g/ml for protocatechuic aldehyde（r=0.999 9）,187.20-2 995.20 μg/ml for salvianolic acid B（r=0.999 7）,23.71-379.39 μg/ml for paeoniflorin（r=0.999 9）and0.20-3.12 μg/ml for ferulic acid（r=0.999 7）;the RSDs of precision,stability and repeatability tests were all less than 2%;the average recoveries were respectively 98.85%（RSD=0.12%,n=6）,97.95%（RSD=0.19%,n=6）,99.18%（RSD=0.37%,n=6）,98.14%（RSD=0.25%,n=6）and 97.16%（RSD=1.36%,n=6）. CONCLUSIONS：The method is simple and convenient with good separation effect,and can be used for the content determination of the 5 components in Xinning tablets.

关 键 词：高效液相色谱法 心宁片 丹参素 原儿茶醛 丹酚酸B 芍药苷 阿魏酸 

分 类 号：R917[医药卫生—药物分析学]