离子色谱法同时测定感冒清热颗粒中9种无机阴离子  

Determination of 9 Inorganic Anions in Ganmao Qingre Granules by Ion Chromatography

作　　者：周伟娥[1] 杨立佼 谢巍[1] 蒋受军 

机构地区：[1]广西中医药大学药学院,南宁530001 [2]广西玉林食品药品检验所,广西玉林537000

出　　处：《中国药房》2015年第18期2546-2549,共4页China Pharmacy

基　　金：广西科学研究与技术开发计划项目(No.桂科攻10124008-15)

摘　　要：目的：建立同时测定感冒清热颗粒中9种无机阴离子（氟离子、甲酸根、氯离子、亚硝酸根、溴离子、硝酸根、硫酸根、草酸根和磷酸根）含量的方法。方法：采用离子色谱法。色谱柱为Ion Pac AS11-HC,流动相为氢氧化钾溶液,等度洗脱（洗脱浓度30mmol/L,洗脱时间15 min）,柱温为室温,进样量为25μl。结果：9种无机阴离子基本达到分离,线性关系良好,相关系数分别在0.999 2~0.999 9范围内;精密度、稳定性、重复性试验的RSD〈3.50%;平均加样回收率分别在92.47%~103.51%范围内,RSD分别在0.42%~3.41%范围内（n=6）。结论：本方法快速、准确、灵敏、重复性好,可用于感冒清热颗粒中9种无机阴离子的测定。OBJECTIVE：To establish the ion chromatography for the mass fractions of 9 inorganic anions（fluoride ion,formate radical,chloride ion,nitrite radical,bromide ion,nitrate radical,sulfate radical,oxalate radical and phosphate radical） in Ganmao qingre granules. METHODS：Ion chromatograph was used. The column was Ion Pac AS11-HC with the mobile phase of potassium hydroxide solution by gradient elution（concentration：30 mmol/L,time：15 min）,the column temperature was room temperature,the volume was 25 μl. RESULTS：The 9 inorganic anions were basically achieved separation and had good linear relationship with r of 0.999 2-0.999 9,respectively;RSDs of precision,stability and reproducibility tests were less than 3.50%. Average recovery was 92.47%-103.51%（RSD=0.42%-3.41%,n=6）,respectively. CONCLUSIONS：The established method is fast,accurate,sensitive and repeatable and can be used for the determination of 9 inorganic anions in Ganmao qingre granules.

关 键 词：离子色谱法 感冒清热颗粒 无机阴离子 

分 类 号：R284.1[医药卫生—中药学]