HPLC法测定加味逍遥丸中芍药苷与甘草苷的含量  

Content Determination of Paeoniflorin and Liquoritin in Jiawei Xiaoyao Pills by HPLC

作　　者：杜蓉[1] 张孟佑[1] 

机构地区：[1]广州军区武汉总医院药剂科,武汉430070

出　　处：《中国药房》2015年第18期2571-2572,共2页China Pharmacy

摘　　要：目的：建立同时测定加味逍遥丸中芍药苷与甘草苷含量的方法。方法：采用高效液相色谱法。色谱柱为Hypersil-ODS C18,流动相为乙腈-水（14∶86,V/V）,流速为1.0 ml/min,检测波长为230 nm,柱温为室温,进样量为10μl。结果：芍药苷、甘草苷质量浓度分别在16.12~80.6、6.08~30.4μg/ml范围内与各自峰面积呈良好的线性关系（r为0.999 6、0.999 8）;精密度、稳定性、重复性试验的RSD〈2.0%;平均加样回收率分别为98.51%、98.08%,RSD分别为1.94%、1.29%（n=6）。结论：该方法操作简单、准确度高,可用于测定加味逍遥丸中芍药苷与甘草苷的含量。OBJECTIVE：To establish the HPLC method for the content determination of paeoniflorin and liquoritin in Jiawei xiaoyao pills. METHODS：It was performed on Hypersil-ODS C18 with the mobile phase of acetonitrile-water（14 ∶ 86,V/V）at the flow rate of 1.0 ml/min,the detection wavelength was 230 nm,the column temperature was room temperature and the volume was 10 μl. RESULTS：The linear range was 16.12-80.6 μg/ml for paeoniflorin（r=0.999 6）and 6.08-30.4 μg/ml for liquoritin（r=0.999 8）;the RSDs of precision,repeatability and stability tests were all less than 2.0%;the average recovery was respectively98.51%（RSD=1.94%,n=6）and 98.08%（RSD=1.29%,n=6）. CONCLUSIONS：The method is simple,accurate and reliable,and can be used for the content determination of paeoniflorin and liquoritin in Jiawei xiaoyao pills.

关 键 词：高效液相色谱法 加味逍遥丸 芍药苷 甘草苷 

分 类 号：R917[医药卫生—药物分析学]