八角莲不定芽的快速培育  

Quick Cultivation of Adventitious Buds From Dysosma Versipellis

作　　者：徐铭泽[1] 陆艳芝 朱应红[1] 刘燕飞[1] 黄碧霞[1] 赵雪艳[1] 吴群英[1] 

机构地区：[1]桂林医学院生物技术学院,广西桂林541004

出　　处：《绿色科技》2015年第6期20-22,共3页Journal of Green Science and Technology

基　　金：国家及广西壮族自治区大学生创新创业训练项目(编号:桂教高教2013[14]号);桂林医学院教学研究与改革委托课题(编号:院教发字[2013]23号);新世纪广西高等教育教改工程项目(编号:2011JGB084)资助

摘　　要：目的探讨八角莲根状茎不定芽快速培育的方法。方法采用L9（33）正交法,对根状茎结节进行浸泡之后,将根状茎栽入培养土中,再用对应的激素水溶液代替自来水进行培养。结果八角莲不定芽快速培育的最佳处理为：选取根状茎第一节结节,BA1.0mg/L＋TDZ0.2mg/L激素水溶液中浸泡24h,不定芽诱导效果最好,每个根状茎诱导的不定芽数可达5~7个。结论用激素水溶液对八角莲根状茎进行浸泡预处理和浇灌,能显著提高八角莲不定芽的产量。The aim of the article is to study the method of quickly cultivating the adventitious buds from rhizomes of dysosma versipellis. Adopting 1.9（33）orthogonal method, the rhizomes are soaked in phytohormone and potted with soil. Then the experiment cultivates the rhizomes with phytohormone solution other than water. The result shows that the best treatment is to chose the first rhizome and soak it in phytohormone solution of BA1.0mg/L＋TDZ0. 2mg/L for 24h, which can induce 5 to 7 adventitious buds from each rhizome. In the end, the article concludes that phytohormone can significantly promote the production of adventitious buds from rhizomes of dysosma versipellis.

关 键 词：八角莲 根状茎 不定芽 快速培育 植物激素 

分 类 号：S567.2[农业科学—中草药栽培]