1株鞭毛抗原减弱沙门菌的α-β位相变异检测  

Report on the α-β phase variation test of a flagellum antigen weakened Salmonella

作　　者：陈燕[1] 徐云龙[1] 

机构地区：[1]杭州市余杭区疾病预防控制中心,浙江杭州311100

出　　处：《中国卫生检验杂志》2015年第11期1811-1812,共2页Chinese Journal of Health Laboratory Technology

摘　　要：目的报道1株从腹泻患者粪便中分离到的同时发生鞭毛抗原减弱和α-β位相变异2种变异的沙门菌。方法将待检沙门菌株划线分离至营养肉汤-营养琼脂平板,并使用相诱导血清进行诱导,使其复原。结果沙门菌株在营养肉汤-营养琼脂平板上传代3代,其第2相H抗原能与相应诊断血清发生反应;使用相诱导血清诱导后,其第1相H抗原能与相应诊断血清发生反应。结论在沙门菌血清分型中应综合考虑各影响因素,特别是H抗原发生自然变异和/或结构受损现象,达到正确分型的目的。Objective To report a Salmonella strain on which flagellum antigen weakening and 2 species of α-β phase varia- tion simultaneously happened. Methods The tested Salmonella strains were placed onto nutrient broth - nutrient agar plate and were induced with phase induction serum for recovery. Results Salmonella strains were aged in nutrient broth - nutrient agar plate 3 generations, and after being induced, the 2nd phase H antigen could react with the corresponding diagnostic serum; the 1st phase H antigens could react with the corresponding diagnostic serum after being induced with phase induction serum. Conclusion Salmonella serotyping should consider all influence factors, especially the H antigens occured natural variation and/or structural damage phenomenon, in order to achieve the purpose of correct serotyping.

关 键 词：沙门菌 血清分型 H抗原 α-β位相变异 

分 类 号：R446.6[医药卫生—诊断学]