HPLC联合UV法测定烟酸片中烟酸的含量  

Determination of the Nicotinic Acid in Nicotinic Acid Tablets by HPLC Combined with UV

作　　者：吴广[1] 曾赛丽[2] 

机构地区：[1]南华大学附属第二医院药剂科,湖南衡阳421001 [2]南华大学附属第二医院呼吸内科

出　　处：《中南医学科学杂志》2015年第3期319-321,共3页Medical Science Journal of Central South China

摘　　要：目的建立一种精确可靠的HPLC/UV法用于测定烟酸片中烟酸的含量。方法：HPLC法采用Aglient C18色谱柱,以0.02 mol/L磷酸二氢钾-乙腈（90∶10）为流动相等度洗脱,流速1.0 m L/min,检测波长263 nm;UV法以磷酸二氢钾-乙腈作为溶剂,在263 nm波长处测定吸光度;并通过检测其线性范围、精密度、准确度和加样回收率对该方法进行验证。结果：烟酸检测质量浓度在5.0~100.0μg/m L范围内,与吸光度和峰面积积分值呈良好的线性关系,UV法检测r=0.9959,平均加样回收率为99.57%,RSD=1.08%（n=6）;HPLC法检测r=0.999 8,平均加样回收率为99.62%,RSD=0.74%（n=6）。结论：HPLC法和UV法联用操作简捷、准确、重复性好,并且具有更好的专属性,可用于烟酸片烟酸的含量测定和质量控制。Objective To establish an accurate and reliable HPLC/UV method for the quantitative determination of nicotinic acid in nicotinic acid tablets. Methods The chromatographic separation conditions employed for HPLV method were chosen using Aglient C18 column. 0.02 moL/L potassium dihydrogen phosphate （Ph 6.8 ）-acetonitrile （90：10 ） were used as the mobile phase pumped at a flow rate of 1.0mL/min and a detection wavelength at 263 nm was used. UV method use potassium dihydrogen phosphate （Ph 6.8）-aeetonitrile （90： 10） as solvent, and the detection wavelength were at 263 nm. The linearity, precision, accuracy, recovery rate and other content were tested to validate the method. Results The linear range of nictinic acid was 5.0 ~ 100.0 Ixg/mL. With UV method （ r = 0. 995 9 ）, the average recovery is 99.57% （ RSD = 1.08 %, n = 6 ） ; with HPLC method （ r = 0.999 8 ）, the average recovery is 99.62% （ RSD = 0.74%, n = 6 ）. Conclusion The HPLC/UV method is simple, accurate,high-specificial and reproducible. This method can be used for the quality control nicotinic acid tablets.

关 键 词：HPLC UV 含量测定 质量控制 烟酸 

分 类 号：R927.2[医药卫生—药学]