阿托伐他汀对模拟失重大鼠肺炎链球菌感染的保护作用  

Protective Effect of Atorvastatin on Streptococcus Pneumoniae Infections in Rats under Simulated Weightless Condition

作　　者：朱敏立[1] 王小辉[1] 王萍[1] 李月越[1] 崔彦[2] 

机构地区：[1]解放军306医院呼吸与重症医学科,北京100101 [2]解放军306医院普通外科,北京100101

出　　处：《解放军医药杂志》2015年第6期6-9,共4页Medical & Pharmaceutical Journal of Chinese People’s Liberation Army

基　　金：全军医学科研"十二五"重点项目(BWS11J051)

摘　　要：目的研究模拟失重状态下阿托伐他汀对上呼吸道定植菌肺炎链球菌导致肺部感染的影响,为航天员呼吸系统保护及感染治疗提供理论依据。方法将Wistar大鼠40只随机分为4组(n=10),分别为失重感染组(weightlessness+infection,WI)、失重感染他汀组(weightlessness+infection+statin,WIS)、对照感染组(control+infection,CI)及对照感染他汀组(control+infection+statin,CIS)。WI和WIS组采用大鼠尾吊法模拟失重状态,CI和CIS组在鼠笼内自由活动,于第3天经气管滴入肺炎链球菌悬液0.4 ml,复制大鼠肺部感染模型。从实验第1天开始他汀组(WIS组、CIS组)给予阿托伐他汀10 mg/(kg·d)药物灌胃,无药物干预组(WI组、CI组)给予等量无菌注射用水灌胃。尾吊8 d后处死动物取材,测血常规、中性粒细胞黏附分子(CD11b/c),肺组织切片观察肺组织病理变化。结果肉眼观察4组大鼠肺组织表面出现不同程度的局部充血及点片状出血;光镜下可见肺泡腔内大量以中性粒细胞为主的炎性细胞浸润,部分肺泡结构消失。上述改变以WI组表现最重,CIS组表现最轻,其中失重大鼠以上叶表现更为突出。各组间白细胞、中性粒细胞值差异有统计学意义(F=3.67,P<0.05;F=7.47,P<0.01),WI组数值最高,CIS组最低。各组间血中性粒细胞CD11b/c比较差异有统计学意义(P<0.01),其中WI组大鼠的CD11b/c值最高(167.75±28.39),CIS组CD11b/c值最低(115.57±18.57)。各组间NEU百分比(NEU%)差异无统计学意义(P>0.05)。结论模拟失重状态下肺炎链球菌所致肺部感染炎性反应更强,病理损害更重,阿托伐他汀对肺组织具有保护作用。Objective To investigate the effect of Atorvastatin on pulmonary infection induced by Streptococcus pneumonia in rats under simulated weightless condition, and to provide the theoretical basis for protection of respiratory system and infection therapy for astronauts. Methods A total of 40 Wistar rats were randomly divided into weightlessness ＋ infection group （WI group, n = 10） , weightlessness ＋ infection ＋ statin group （WIS group, n = 10） , control ＋ infection group （CI group, n = 10） and control ＋ infection ＋ statin group （CIS group, n = 10）. The models of simulated weightlessness were established in WI and WIS groups, while the rats in CI and CIS groups were fed in mouse cages with free running space. Bacterial pneumonia models were established in the 4 groups after the rats had been fed for 3 d. WIS and CIS groups were treated with Atorvastatin 10 mg/（ kg ·d） via intragastric administration on the first experiment day, while WI and CI groups were treated with same volume of sterile water via intragastric injection. The rats were killed at the end of tail-suspension for 8 d. The routine blood test and level of CD11b/c of neutrophilic granulocyte in the blood samples were detected, and the pathological changes of lung tissues in slices were observed. Results In the four groups, local congestion and spot-film hemorrhage in different degrees were found in the surfaces of lung tissues by macroscopic observation; inflammatory cells were infiltrated in the interstitial alveolar space which was mainly a large number of neutrophils under the microscope, and some parts of constructions in the alveolar space disappeared. All of the above pathological changes showed that WI group was the severest, and CIS group was the least, and the changes were more prominent in superior lobe of rats under simulated weightlessness. There were significant differences in White blood cells and neutrophilic grannlocyte counts in the four groups （ F = 3.67, P 〈 0. 05 ; F = 7.47, P 〈 0. 01 ）, and the

关 键 词：失重模拟 大鼠 Wistar 阿托伐他汀 肺炎链球菌 

分 类 号：R856[医药卫生—航空、航天与航海医学]