脊髓多巴胺D2受体在大鼠神经病理性痛中的作用  

Role of spinal dopamine D2 receptors in a rat model of neuropathic pain

作　　者：冷鑫[1] 隽立芹 马正良[1] 夏小萍[1] 

机构地区：[1]南京大学医学院附属鼓楼医院麻醉科,210008

出　　处：《中华麻醉学杂志》2015年第3期330-332,共3页Chinese Journal of Anesthesiology

基　　金：基金项目：国家自然科学基金（81171048）;江苏省麻醉学重点学科基金项目（XK201140）;南京市卫生人才项目（YKK08072）

摘　　要：目的评价脊髓多巴胺D：受体在大鼠神经病理性痛中的作用。方法健康雄性SD大鼠30只，6—8周龄，体重180—200g，采用随机数字表法，将其分为5组（／2=6）：正常对照组（c组）、假手术组（s组）、神经病理性痛组（NP组）、生理盐水组（N组）和多巴胺D，受体激动剂喹吡罗组（Q组）。采用坐骨神经慢性压迫性损伤法建立大鼠神经病理性痛模型。于术后7d时，N组经30s鞘内注射生理盐水10μl，Q组经30s鞘内注射多巴胺D：受体激动剂喹吡罗10μg（溶于10μl生理盐水中）。于术前1d、术后3和7d、给药后30min、1、2、4、8和16h（T。）时，测定机械缩足反应阈（MWT）和热缩足潜伏期（TwL）。结果C组和S组各时点MWT和TwL比较差异无统计学意义（P〉0．05）；与C组和S组比较，NP组、N组和Q组T1-8时MWT降低，TWL缩短（P〈0．05）；与NP组比较，N组各时点各指标差异无统计学意义（P〉0．05），Q组T4-6时MWT升高，TWL延长（P〈0．05）。结论脊髓多巴胺D2受体功能抑制参与了大鼠神经病理性痛的维持。Objective To evaluate the role of spinal dopamine D2 receptors in a rat model of neuropathic pain. Methods Thirty healthy male Sprague-Dawley rats, aged 6-8 weeks, weighing 180- 200 g, were randomly divided into 5 groups （ n = 6 each） using a random number table： control group （group C） , sham operation group （group S） , neuropathic pain group （group NP） , normal saline group （group N） and dopamine D2 receptor agonist quinpirole group （group Q）. Neuropathic pain was produced by chronic constriction injury of the sciatic nerve （CCI） in rats anesthetized with intraperitoneal 2% pentobarbital sodium 40 mg/kg. At 7 days after CCI, normal saline 10μl was injected intrathecally over 30 s in group N, and quinpirole 10 p,g （in 10μl of normal saline） was injected intrathecally over 30 s in group Q. At 1 day before CCI, 3 and 7 days after CCI, and 30 min and 1, 2, 4, 8 and 16 h after administration, mechanical paw withdrawal threshold （MWT） and thermal paw withdrawal latency （TWL） were measured. Results There was no significant difference in MWT and TWL at each time point between group C and group S. MWT was significantly lower, and TWL was shorter at T1-8.s in NP, N and Q groups than in C and S groups. Compared with group N, no significant change was found in MWT and TWL at each time point in N group, and MWT was significantly increased, and TWL was prolonged at T4_6 in group Q. Conclusion Inhibited function of spinal dopamine D2 receptors is involved in the maintenance of neuropathic pain in rats.

关 键 词：受体 多巴胺D2 脊髓 神经痛 

分 类 号：R614[医药卫生—麻醉学]