Src激酶在内毒素血症小鼠肝损伤中的作用  

Role of Src kinase in liver injury in endotoxemic mice

作　　者：段家翔 甯交琳[1] 陈林[1] 陈倩[1] 袁碧英 鲁开智[1] 

机构地区：[1]第三军医大学附属西南医院手术麻醉科,重庆市400038

出　　处：《中华麻醉学杂志》2015年第3期355-357,共3页Chinese Journal of Anesthesiology

基　　金：基金项目：国家自然科学基金青年科学基金（81100055）

摘　　要：目的评价Src激酶在内毒素血症小鼠肝损伤中的作用。方法雌性BABL／c小鼠48只，体重15—20g，3～4月龄，采用随机数字表法分为3组（／Z=16）：对照组（c组）、内毒素血症组（LPS组）和Src激酶抑制剂PP2组（PP2组）。LPS组和PP2组腹腔注射脂多糖（LPS）20mg／kg，C组给予等容量PBS，2h后PP2组腹腔注射PP21mg／kg。于注射LPS或PBS后6h取8只大鼠，经腹主动脉采集血样，测定血清碱性磷酸酶（ALP）水平；随后处死大鼠，取肝组织，检测核因子E2相关因子2（Nrf2）水平、超氧化物歧化酶（SOD）活性、丙二醛（MDA）含量及髓过氧化物酶（MPO）活性；于注射LPS或PBS后24h处死8只大鼠，取肝组织，行病理学观察。结果与c组比较，LPS组和PP2组血清ALP、肝组织MDA和MPO的水平升高，SOD和Nrf2的水平降低（P〈0．05）；与LPS组比较，PP2组血清ALP、肝组织MDA和MPO的水平降低，SOD和Nrf2的水平升高（P〈0．05）。PP2组小鼠肝组织病理学损伤较LPS组减轻。结论Src激酶参与了内毒素血症诱发小鼠肝损伤的过程。Objective To evaluate the role of Src kinase in liver injury in endotoxemic mice. Methods Forty-eight female BABL/c mice, aged 3-4 months, weighing 15-20 g, were randomly divided into 3 groups （n = 16 each） using a random number table： control group （C group） , endotoxemia group （lipopolysaceharide （LPS） group） and Src kinase inhibitor PP2 group （PP2 group）. Endotoxemia was in- duced by intraperitoneal LPS 20 mg/kg in LPS and PP2 groups, while the equal volume of PBS was given in group C. In PP2 group, PP2 1 mg/kg was injected intraperitoneally at 2 h after LPS administration. At 6 h after LPS or PBS injection, 8 mice in each group were chosen, and blood samples were collected from the abdominal aorta for determination of the serum levels of alkaline phosphatase （ALP）. The mice were then sacrificed and livers were removed for determination of nuclear factor E2-related factor 2 （Nrf2） level, superoxide dismutase （SOD） activity, malondialdehyde （MDA） content and myeloperoxidase （MPO） ac- tivity in liver tissues. The other 8 mice in each group were sacrificed at 24 h after LPS or PBS injection, and the livers were harvested for examination of pathological changes. Results Compared with C group, the serum levels of ALP and MDA content and MPO activity in liver tissues were significantly increased, and SOD activity and Nrf2 levels in liver tissues were decreased in LPS and PP2 groups. Compared with LPS group, the serum levels of ALP and MDA content and MPO activity in liver tissues were significantly decreased, and SOD activity and Nrf2 levels in liver tissues were increased in PP2 group. The pathological changes of liver tissues were significantly attenuated in PP2 group as compared with LPS group. Conclusion Src kinase is involved in endotoxemia-induced liver injury in mice.

关 键 词：src族激酶类 内毒素血症 肝损伤 

分 类 号：R614[医药卫生—麻醉学]