川芎嗪对高转移性人肺巨细胞癌PG-BE1干细胞样细胞黏附能力的影响  

Effect of Ligustrazine on the Adhesion of PG-BE1 Stem Like Cells in the Pulmonary Giant Cell Carcinoma of Human with High Metastatic Lung Cancer

作　　者：杨栋[1] 张培彤[1] 王耀焓[1] 郭秀伟[1] 马雪曼 

机构地区：[1]中国中医科学院广安门医院 [2]北京中医药大学

出　　处：《中医杂志》2015年第13期1140-1144,共5页Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金(81173450)

摘　　要：目的研究川芎嗪对高转移性人肺巨细胞癌PG-BE1干细胞样细胞黏附能力的影响及可能作用机制。方法采用无血清成球培养法分选PG-BE1细胞中的干细胞样细胞,并检测其表面OCT-4、SOX-2、Nanog蛋白表达及平板克隆形成实验进行验证。再采用人造基底膜黏附实验比较PG-BE1干细胞样细胞在不同环境(常氧和低氧环境)与其原代细胞黏附能力的差异,同时观察不同浓度川芎嗪(终浓度分别为100、300、500μg/ml)及顺铂(2μg/ml)干预后各组干细胞样细胞的黏附能力,以及干预后各组干细胞样细胞表面黏附分子β整合素、CD44V6的表达量。结果与原代细胞相比,PG-BE1干细胞样细胞克隆灶形成能力更强,干细胞样细胞表面的SOX-2、OCT-4及Nanog蛋白的表达相对光密度更强(P<0.05或P<0.01)。常氧干细胞样细胞和低氧干细胞样细胞黏附能力均明显高于原代细胞(P<0.05或P<0.01),川芎嗪中、高剂量组及顺铂组干细胞样细胞黏附能力低于对照组(P<0.01),川芎嗪高剂量组细胞黏附抑制率最高达42.2%。常氧干细胞样细胞和低氧干细胞样细胞β整合素、CD44V6表达量均高于原代细胞(P<0.01),川芎嗪低、中、高剂量组及顺铂组β整合素表达均较对照组下调(P<0.01),川芎嗪高剂量组CD44V6表达较对照组亦明显降低(P<0.01)。结论 PG-BE1干细胞样细胞对基底膜的黏附能力强于其原代细胞,川芎嗪可抑制其黏附能力,其机制可能与抑制细胞表面β整合素、CD44V6蛋白的表达有关。Objective To study the effect and the possible mechanism of ligustrazine on the adhesion of PG-BE1 stem like cells in the pulmonary giant cell carcinoma of human with high metastatic lung cancer. Methods The stem like cells were sorted from PG-BE1 cells by no serum ball culture,the expression of OCT-4,SOX-2,Nanog protein and the flat panel clone formation experiment were tested for verification. Then artificial basement membrane adhesion experiment was taken to compare the difference of adhesion between PG-BE1 stem like cells and its primary cells in different environments（ normal oxygen and hypoxia）. At the same time,the adhesion ability of stem like cells in each group with intervention of different concentrations of ligustrazine（ final concentrations of 100,300,500 g / ml）and cisplatin（ 2 g / ml） were observed,the surface adhesion molecules beta integrin and the expression of CD44V6 in the stem like cells in each group were also observed after intervention. Results Compared with the primary cells,the PG-BE1 stem cells like cells were more capable of forming clone foci. The expression of SOX-2 OCT-4 and Nanog protein in the surface of stem like cells was stronger（ P〈0. 05 or P〈0. 01）. The adhesion ability of the normal oxygen stem like cells and the hypoxia stem like cells was significantly higher than that of the primary cells（ P〈0. 5or P〈0. 01）. The adhesion ability of the stem like cells in ligustrazine groups with middle and high dose and cisplatin groups was lower than that in control group（ P〈0. 01）. The rate of cell adhesion inhibition was up to 42. 2% in the ligustrazine group with high dose. The expression of beta integrin and CD44V6 in the stem like cells of normal oxygen and hypoxia was higher than those of the primary cells（ P〈0. 01）. The expression of beta integrin in groups of ligustrazine with low,middle and high dose and cisplatin group was lower than that of control group（ P〈0. 01）. The expression of CD44V6 in the high dose group of ligustrazine was

关 键 词：川芎嗪 人肺巨细胞癌 肿瘤干细胞 细胞黏附 β整合素 CD44V6 

分 类 号：R285[医药卫生—中药学]