毛蕊异黄酮上调过氧化物酶体增殖因子活化受体γ抑制大鼠肝星状细胞活化的研究  

Study on inhibitory effect of calycosin on hepatic stellate cell activation in rats by up-regulating peroxisome proliferator-activated receptor γ

作　　者：平键[1,2] 陈红云[3] 周扬[1] 陈高峰[1] 徐列明[1] 成扬[1,4] 

机构地区：[1]上海中医药大学附属曙光医院,上海201203 [2]上海市中医临床重点实验室,上海201203 [3]第二军医大学长海中医医院,上海200433 [4]上海市浦东新区传染病医院,上海201299

出　　处：《中国中药杂志》2015年第12期2383-2388,共6页China Journal of Chinese Materia Medica

基　　金：上海市自然科学基金项目(10ZR1430700);上海市浦东新区科委项目(PKJ2014-Y37);上海市中医药三年行动计划项目(ZYSNXD)

摘　　要：该研究观察了毛蕊异黄酮对大鼠原代肝星状细胞(HSC)增殖和活化的影响,并探讨其是否通过影响核因子过氧化物酶体增殖因子活化受体γ(PPARγ)和法尼酯衍生物X受体(FXR)发挥作用。结果发现毛蕊异黄酮能抑制HSC的增殖,抑制活化标志物α-平滑肌肌动蛋白和Ⅰ型胶原的表达;随着HSC活化时间的延长,FXR表达逐渐降低,但毛蕊异黄酮对FXR表达没有明显影响;毛蕊异黄酮可浓度依赖性地上调PPARγ的表达及核转位,PPARγ拮抗剂可阻断毛蕊异黄酮对HSC活化的抑制作用。因此,结论认为毛蕊异黄酮可抑制HSC增殖和活化,其作用不通过FXR而是与上调PPARγ信号通路有关。To observe the effect of calycosin on the proliferation and activation of primary hepatic steliate cells （HSCs） in rats, and prove calycosin shows the effects through peroxisome proliferator-activated receptor γ（PPARγ） and farnesoid X receptor （FXR）. The results indicated that calycosin could inhibit HSC proliferation and expressions of activation marker smooth muscle actin-α and type Ⅰ collagen. With the increase in HSC activation time, FXR expression reduced, hut with no notable impact from calycosin. Calycosin could up-regulate PPART expression and its nuclear transition in a concentration-dependent manner. Its prohibitory effect on HSC activation could be blocked by PPART antagonist. In conclusion, calycosin could inhibit HSC activation and proliferatian, which may be related with the up-regulation of PPART signal pathway.

关 键 词：毛蕊异黄酮 肝星状细胞 过氧化物酶体增殖因子活化受体 

分 类 号：R285[医药卫生—中药学]