丽格海棠叶片优化培养高效再生体系的研究  

Research of optimization of culture and high efficient regeneration system for Begonia × Elatior(balamon) using leaf blade

作　　者：雷颖[1] 任俞新 吴利红[1] 

机构地区：[1]甘肃林业职业技术学院园林工程系,甘肃天水741020

出　　处：《农业科学研究》2015年第2期91-93,共3页Journal of Agricultural Sciences

基　　金：甘肃林院自然科研项目(GSLY-201510)

摘　　要：以丽格海棠叶片为外植体,进行不定芽的诱导和植株再生试验研究,结果表明:叶切块可直接分化不定芽,以MS+6-BA2.0 mg/L+TDZ0.1 mg/L+NAA0.5 mg/L是较适合的分化培养基,分化率达100%,平均芽数8.1;不定芽增殖以MS+6-BA 1.0 mg/L+KT0.5 mg/L+NAA0.2 mg/L效果较好,平均增殖倍数为12.2左右,生根培养基以1/2MS+NAA0.1 mg/L+IBA0.3 mg/L效果较好,平均生根数为4.4条左右,生根率为100%;瓶苗移栽于森林土∶草碳=(1∶1)的基质中生长良好,成活率达98%以上.The leaf blade of Begonia x Elatior （balamon） was used as explants to research on adventitious bud induction and plantlet regeneration. The results showed that：cut of Leaves can be directly differentiation of adventitious buds the suitable differential medium for adventitious buds induction was MS＋6-BA2.0 mg·L-1＋TDZ0.1 mg·L-1＋NAA0.5 mg·L-1 the differentiation rate and mean number of shoots could reach to 100% and8.1; it could effectively improve the proliferation of adventitious buds for MS＋6-BA 1.0 mg·L-%KT0.5 mg·L-1＋NAA0.2 mg·L-1 and obtained a higher proliferation rate of 12.2, the optimal rooting medium was 1/2MS＋NAA0.1mg·L-I＋IBA0.3mg·L-1 with its average survival rate and root number up to100%,4.4 respectively; the test-tube plantlets were easy to be transplanted and survive when it transplanting medium was forest soils ： Substratum=1 ： 1 , and showed 98% survival rate.

关 键 词：丽格海棠 叶片 优化培养 高效再生 

分 类 号：Q813.11[生物学—生物工程]