香烟提取物对COS-7细胞表面血栓调节蛋白荧光表达的影响  

Influence of cigarette smoking extract on fluorescence expression of thrombomodulin on surface of COS-7 cells

作　　者：魏玉杰[1] 刘惠亮[1] 李屹[1] 赖斌[1] 

机构地区：[1]中国人民武装警察部队总医院心内科,北京100039

出　　处：《心血管康复医学杂志》2015年第3期243-246,F0003,共5页Chinese Journal of Cardiovascular Rehabilitation Medicine

基　　金：国家自然科学基金资助项目(81370315)

摘　　要：目的:探讨香烟提取物(CSE)是否对非洲绿猴肾成纤维细胞(COS-7)表面血栓调节蛋白(TM)荧光蛋白表达的影响。方法:成功构建TM-绿色荧光蛋白(GFP)质粒后,使其转染至COS-7细胞,用制备好的5%CSE孵育(5%CSE组);将无血清最低必需培养基(MEM,单纯培养基)加入一定体积的PBS进行同步培养作为对照组;采用流式细胞仪计数方法检测不同时间点COS-7表面TM荧光蛋白表达量的变化。结果:与对照组比较,5%CSE在1h、6h对COS-7表面TM荧光蛋白表达没有明显变化[1h:(134.99±18.41)比(146.61±12.06),6h(116.89±27.28)比(123.89±39.24),P均>0.05]。结论:5%香烟提取物对非洲绿猴肾成纤维细胞表面血栓调节蛋白荧光表达没有影响。Objective：To explore whether cigarette smoking extract （CSE） has influence on fluorescence protein ex-pression of thrombomodulin （TM） on surface of African green monkey kidney fibroblast cells （COS-7） or not . Methods：When TM-green fluorescent protein （GFP） plasmid was successfully constructed ,COS-7 cells were trans-fected by it ,then incubated by prepared 5% CSE （5% CSE group） ,PBS of certain volume was added to serum-free minimal essential medium （MEM ,simple medium） ,which was cultured at the same time and treated as control group .Flow cytometry counting method was used to detect change of TM-GFP expression amount on COS-7 surface at different time point .Results：Compare with control group ,there were no significant difference in the expression of TM-GFP on COS-7 surface at 1h and 6h in 5% CSE group [1h ：（134.99 ± 18.41） vs .（146.61 ± 12.06） ,6h ：（116.89 ± 27.28） vs .（123.89 ± 39.24） ,P〉0.05 both] .Conclusion：The 5% cigarette smoking extract has no influ-ence on fluorescence expression of thrombomodulin on surface of African green monkey kidney fibroblast cells .

关 键 词：烟草 血栓调节蛋白 COS细胞 绿色荧光蛋白质类 

分 类 号：R543.09[医药卫生—心血管疾病]