慢性乙型肝炎患者白细胞介素33水平变化研究  

Study on Changes of Interleukin-33 Levels in Patients with Chronic Hepatitis B

作　　者：李华[1] 张素贞[1] 贾倩[1] 李梦嘉[1] 阎敏娜 贾晓晖[2] 

机构地区：[1]河北北方学院附属第一医院检验科,河北张家口075000 [2]河北北方学院医学检验学院,河北张家口075000

出　　处：《医学综述》2015年第11期2081-2083,共3页Medical Recapitulate

摘　　要：目的检测阿德福韦酯治疗前后慢性乙型肝炎患者血清中白细胞介素33（IL-33）水平变化,探讨其临床意义。方法选取2012年10月至2013年3月于河北北方学院附属第一医院进行诊治的89例慢性乙型肝炎患者作为慢性乙型肝炎组,89例在本院体检确定健康者作为健康对照组,应用全自动生化分析仪和荧光定量聚合酶链反应（PCR）仪于不同时相点检测血清中丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）水平及乙型肝炎病毒（HBV）DNA荷载量状态,同时应用酶联免疫吸附测定方法检测所有研究对象血清中IL-33、干扰素γ（IFN-γ）、肿瘤坏死因子α（TNF-α）、IL-2、IL-4、IL-6和IL-10水平。结果慢性乙型肝炎组治疗前IL-33、ALT、AST水平及HBV DNA拷贝量显著高于健康对照组[57.1（23.7-138.5）ng/L比20.5（9.3-67.5）ng/L;129.3（28.3-702.5）U/L比17.4（9.1-23.2）U/L;97.5（31.4-327.1）U/L比16.2（5.9-27.8）U/L;7.8（5.6-9.8）×10^3log10拷贝/L比1.1（0.3-1.7）×10^3log10拷贝/L],差异有统计学意义（P〈0.01）,且IL-33与ALT和AST无显著相关性（P〉0.05）,与HBV DNA拷贝量正相关（r=0.383,P〈0.05）;治疗后慢性乙型肝炎组IFN-γ、IL-2、IL-10和IL-6水平较治疗前显著升高[12.4（10.8-16.1）ng/L比6.7（2.1-12.0）ng/L;9.0（4.4-14.1）ng/L比5.3（3.6-9.5）;7.1（5.8-13.1）ng/L比6.6（4.3-11.4）ng/L;7.1（6.3-10.7）ng/L比6.5（5.7-8.8）ng/L,P〈0.05或P〈0.01],TNF-α和IL-4水平变化差异无统计学意义,均与IL-33无相关性（P〉0.05）。结论IL-33在慢性乙型肝炎患者免疫调节中具有一定作用,可能是HBV相关肝损伤中一个病理因素,检测患者血清中IL-33水平对于免疫状态监控具有重要意义。Objective To explore the changes of serum interleukin-33 （ IL-33 ） levels in patients with chronic hepatitis B before and after adefovir dipivoxil therapy and its clinical significance.Methods A total of 89 patients with chronic hepatitis B admitted in the First Affiliated Hospital of Hebei North University from Oct.2012 to Mar.2013 were selected as the chronic hepatitis B group,and another 89 healthy people under-went physical examination in the hospital during the same period were selected as the healthy control group.Automatic biochemical analyzer and fluorescence quantitative polymerase chain reaction （ PCR） were applied to detect serum levels of alanine transaminase（ ALT） and aspertate aminotransferase（ AST） and the quantity of hepatitis B virus（ HBV） DNA load at different phases,and enzyme linked immunosorbent assay （ELISA） method was used to detected serum levels of IL-33,interferon-γ（IFN-γ）,tumor necrosis factor-α（TNF-α）,IL-2,IL-4,IL-6 and IL-10.Results In chronic hepatetes B group,levels of IL-33,ALT,AST and HBV DNA copies before treatment were significantly higher than those of the healthy control group [ 57.1 （23.7-138.5） ng/L vs 20.5（9.3-67.5） ng/L;129.3（28.3-702.5）U/L vs 17.4（9.1-23.2）U/L;97.5 （31.4-327.1）U/L vs 16.2（5.9-27.8）U/L; 7.8 （5.6-9.8） ×10^3 log10 copies/L vs 1.1（0.3-1.7） × 10^3 log10 copies/L],the differences were statistically significant（P〈0.01）,while there was positive corre-lation between IL-33 level and HBV DNA load（r=0.383, P〈0.05）.Serum lveels of IFN-γ, IL-2, IL-10 and IL-6 in chronic hepatitis B group significantly increased after treatment [ 12.4 （ 10.8-16.1 ） ng/L vs 6.7 （2.1-12.0） ng/L;9.0（4.4-14.1） ng/L vs 5.3（3.6-9.5）;7.1（5.8-13.1） ng/L vs 6.6 （4.3-11.4） ng/L;7.1（6.3-10.7） ng/L vs 6.5（5.7-8.8） ng/L,P〈0.05 or P 〈0.01],changes of TNF-αand IL-4 levels showed no statistically significant differences,and there were no correlation of IL-33 level with TNF-αand IL-4

关 键 词：慢性乙型肝炎 白细胞介素33 阿德福韦酯 肝功能 

分 类 号：R512.62[医药卫生—内科学]