创新型诺如病毒GⅠ/GⅡ抗原荧光纳米颗粒快速联检试纸条检测效能测评  

Evaluation of the Detection Performance of Innovative Designed Norovirus GⅠand GⅡ Antigen Fluorescent Nanoparticles Rapid Combined Detection Test Strip

作　　者：李琛[1] 廖焕金 兰巧芬 盛慧英 叶玲[2] 李亚宁[2] 王刚[2] 潘庆军[2] 

机构地区：[1]广东医学院附属医院检验科,广东湛江524001 [2]广东医学院附属医院临床医学研究中心,广东湛江524001 [3]广州市妇女儿童医疗中心中心实验室,广东广州510623

出　　处：《标记免疫分析与临床》2015年第6期577-580,共4页Labeled Immunoassays and Clinical Medicine

基　　金：国家自然科学基金(81202346);广东省卫生厅(A2014481)

摘　　要：目的评估诺如病毒GⅠ和GⅡ抗原荧光纳米颗粒快速联检试纸条的灵敏度和特异性。方法以培养的轮状病毒等其他肠道病原体和245例具有胃肠道感染症状患者的粪便为检测标本,分别用诺如病毒GⅠ和GⅡ抗原荧光纳米颗粒快速联检试纸条和诺如病毒GⅠ和GⅡ抗原二合一彩色微球法检测试纸条检测。结果诺如病毒GⅠ和GⅡ抗原荧光纳米颗粒快速联检试纸条的灵敏度显著高于诺如病毒GⅠ和GⅡ抗原二合一彩色微球法检测试纸条;对常见的轮状病毒(Wa株)、肠道腺病毒(40型)、肠道病毒柯萨奇病毒(A16)、埃可病毒(30型)和肠道病毒(EV71)无交差反应。结论诺如病毒GⅠ和GⅡ抗原的荧光纳米颗粒快速联检试纸条灵敏度显著高于彩色微球法快速检测试纸条,特异性良好。Objective To evaluate the sensitivity and specificity of Norovirus GⅠ and GⅡ antigen fluorescent nanoparticles rapid combined detection test strip. Methods The samples including cultured rotavirus and 245 specimens of patients with symptoms of gastrointestinal infection were collected. The fluorescent nanoparticles rapid combined detection test strip and colored microspheres rapid detection test strips were used to detect the same samples and the performance of two detection methods were analyzed. Results The sensitivity of fluorescent nanoparticles rapid combined detection test strip was significantly higher than colored microspheres rapid test strip and no specific reaction with rotavirus （ Wa strain ）, adenovirus （ type 40 ）, coxsachievirus （ type A16 ）, ECHO virus （ type 30 ） and enterovirus （ type 71 ）. Conclusion The sensitivity and specificity of fluorescent nanoparticles rapid combined detection test strips are significantly higher than colored microspheres rapid detection test strip, and suitable for clinical application.

关 键 词：诺如病毒 GⅠ和GⅡ抗原 荧光纳米颗粒 彩色微球 灵敏度 特异性 

分 类 号：R446.6[医药卫生—诊断学]