复方薤白胶囊对慢性阻塞性肺疾病大鼠模型气道重塑作用的研究  

The Study of the Role on of CMOC on the Airway Remodeling in Rats with COPD

作　　者：袁永丰[1] 曾亮[2] 

机构地区：[1]南京中医药大学附属医院,江苏南京210029 [2]南京中医药大学第一临床医学院,江苏南京210046

出　　处：《中国中医急症》2015年第6期967-968,1032,共3页Journal of Emergency in Traditional Chinese Medicine

基　　金：江苏省高校自然科学研究面上资助项目(11KJB360002)

摘　　要：目的观察复方薤白胶囊对慢性阻塞性肺疾病(COPD)大鼠气道重塑作用的影响。方法120只大鼠采用随即机表分为正常对照组、模型组及复方薤白治疗组3组,气管注射脂多糖及熏香烟联合造模方法制备COPD大鼠模型。正常对照组、模型组灌胃10 m L/kg纯净水,复方薤白治疗组灌胃给予复方薤白胶囊混悬液(38 g/m L),连续14 d。光镜下观察大鼠病理学改变;免疫组化法测量支气管-肺组织中TIMP-1、MMP-9蛋白阳性表达面积比率。结果模型组支气管纤毛上皮部分脱落,纤毛粘连倒伏、变性、坏死,气道上皮复层化,杯状上皮细胞增生;支气管平滑肌厚度明显增加,管腔内有中度渗出物;气管黏膜下层、肌层可见大量嗜酸性细胞、淋巴细胞、巨噬细胞及少量浆细胞浸润。复方薤白治疗组支气管纤毛轻度粘连倒伏、变性,杯状上皮细胞轻度增生,支气管平滑肌厚度轻度增加,管腔内无渗出物;气管黏膜下层、肌层可见少量嗜酸性细胞、淋巴细胞及少量浆细胞浸润。与正常对照组比较,模型组大鼠支气管壁厚度、平滑肌厚度、MMP-9、TIMP-1阳性细胞平均面积、累积光密度、光密度均高于正常对照组(均P<0.05)。与模型组比较,复方薤白治疗组大鼠支气管壁厚度、平滑肌厚度、MMP-9、TIMP-1阳性细胞平均面积、累积光密度、光密度则低于模型组(均P<0.05)。结论复方薤白胶囊可以有效减轻COPD支气管壁及平滑肌增厚,有效降低COPD大鼠支气管-肺组织TIMP-1、MMP-9的分布及表达,抑制COPD大鼠模型的气道重塑,延缓COPD的发展。Objective： To investigate the role of CMOC on the in airway remodeling in rats with COPD. Meth- ods： The rat model of COPD was established by cigarette smoking combined with intratracheal injection of lipopolysaccharide （LPS）. 120 rats were randomly divided into three groups ：normal control group,model group and CMOC treatment group. The normal group and model group were intragastrically given the 10 mL/kg pure wa- ter,the treatment group were given CMOC by stomach （38 g/mL）,for 14 consecutive days. Pathological changes were observed under light microscopy;positive expressions of MMP-9 and TIMP-1 area ratios in the bronchial- lung tissue were determined using immunohistochemical method. Results： The bronchial wall and smooth muscle layer in the model group were thicker than normal group. Expression of MMP-9 and TIMP-1 in the model group were higher than normal group,the differences were statistically significant;compared with model group,the ex- pression of MMP-9 and TIMP-1 of CMOC in treatment group were significantly decreased （all P〈 0.05）. Conclu- sion： CMOC can effectively improve the bronchial wall and smooth muscle layer thickening, CMOC can effective- ly reduce the expression and distribution of MMP-9,TIMP-1 ,inhibit the airway remodeling of COPD rats model and delay the development of COPD.

关 键 词：COPD 大鼠模型 气道重塑 复方薤白胶囊 

分 类 号：R285.5[医药卫生—中药学]