碳纳米粒的制备及其用于氯霉素的测定  

Preparation and application of carbon dots in chloramphenicol determination

作　　者：严拯宇[1,2] 舒娟[1,2] 余雁[1,2] 张正伟[1,2] 唐璐[1,2] 陈建秋[1,2] 

机构地区：[1]中国药科大学药物质量与安全预警教育部重点实验室,南京210009 [2]中国药科大学理学院分析化学教研室,南京210009

出　　处：《中国药科大学学报》2015年第3期322-327,共6页Journal of China Pharmaceutical University

基　　金：江苏省普通高校研究生科研创新计划资助项目(No.KYZZ-0185);国家级大学生创新创业训练计划资助项目(No.J1030830)~~

摘　　要：首次以柠檬酸为碳源、甘氨酸为修饰剂,通过高温热解法一步合成修饰碳纳米粒。经过正丁醇萃取纯化,碳纳米粒的粒径更加均一,荧光强度更大,且性能得到了改善。最终制得的碳纳米粒呈棕黄色,在380 nm激发波长下,最大发射波长出现在480 nm附近,其荧光量子产率高达47%。氯霉素对碳纳米粒的荧光具有显著的猝灭效应,并呈现一定的规律性,据此建立了对氯霉素含量测定的新方法。该方法简便快捷,易于操作,线性关系良好（r=0.999 7）,回收率在99%~101%（RSD=0.3%）,显示了碳纳米粒在药物检测方面的潜在应用前景。Carbon dots （CDs） were firstly prepared via a simple pyrolysis route using citric acid as the carbon precursor and glycine as modifier. The obtained CDs were further purified by n-butyl alcohol, which made the particle size more uniform and the fluorescence intensity stronger. When excited at 380 nm, the maximum emis- sion wavelength of the CDs was about 480 nm, with a quantum yield of 47%. Based on fluorescence quenching, a novel method for chloramphenicol assay was developed with glycine-passivated CDs. This method is simple and rapid. A linear relationship between the change of fluorescence intensity and the concentration of chloramphenicol was obtained with a relation coefficient of 0. 999 7. The recovery was in the range of 99% to 101% with a relative standard deviation of 0. 3%, which shows CDs＇ potential application in drug detection.

关 键 词：碳纳米粒 柠檬酸 氯霉素 荧光猝灭 含量测定 

分 类 号：R943[医药卫生—药剂学]