亚慢性砷暴露对小鼠精子发育及Uty基因表达影响  

Effect of subchronic exposure to arsenic on sperm production and Uty gene expression in mice

作　　者：赵连[1] 孟军[1] 孙亚慧[1] 朴丰源[2] 李双月[2] 

机构地区：[1]大连市疾病预防控制中心,辽宁116021 [2]大连医科大学公共卫生学院

出　　处：《中国公共卫生》2015年第6期751-753,共3页Chinese Journal of Public Health

基　　金：国家自然科学基金(30571584;81102160)

摘　　要：目的探讨亚慢性砷暴露对小鼠精子发育和调控基因Uty表达影响。方法雄性小鼠随机分为对照组、三氧化二砷(As2O3)1、2、4 mg/L暴露组,每组10只,自然饮用含不同浓度As2O3水染毒,连续暴露60 d。取双侧睾丸、附睾,称重计算脏器系数,显微镜下观察精子活动度,苏木素-伊红染色观察精子畸形率和生精周期各级生精细胞数量,实时定量PCR检测Y染色体Uty基因表达。结果对照组、1、2、4 mg/L染砷组小鼠睾丸脏器系数分别为(3.6±0.41)、(3.47±0.41)、(3.15±0.46)、(3.14±0.45),附睾脏器系数分别为(0.82±0.14)、(0.80±0.12)、(0.70±0.11)、(0.68±0.11),与对照组比较,2、4 mg/L染砷组小鼠睾丸、附睾脏器系数明显降低(P<0.05);2、4 mg/L染砷组小鼠精子活动度分别为82%、81%,明显低于对照组(97%),差异有统计学意义(P<0.05),4 mg/L染砷组小鼠精子畸形率(6.6%)明显高于对照组(2.6%)(P<0.05)。与对照组比较,4 mg/L染砷小鼠睾丸中A型精原细胞、前细线期精母细胞、中粗线期精母细胞、第七阶段精细胞数量[分别为(33.4±3.8)%、(4.0±4.1)%、(125±1.2)%、(220±18.8)%]明显下降(P<0.05);与对照组比较,2、4 mg/L砷染毒小鼠睾丸和附睾内Uty基因表达下降,差异均有统计学意义(P<0.05)。结论亚慢性砷暴露影响小鼠精子发育,其雄性生殖毒性作用可能与Uty基因表达下调有关。Objective To explore the effect of subchronic exposure to arsenic on sperm production and Uty gene expression in mice. Methods Forty male mice were equally divided into 4 groups randomly. The mice in group A re- ceived water alone （control）, group B, C, and D received 1,2, and 4 mg/L arsenic trioxide （As2 03 ）in drink water, respec- tively. After 60 days, the mice were sacrificed. The organ coefficients of testis and epididymis were determined. The sperm activity,quality, and spermatogenesis were observed. Furthermore,the expressions of Uty gene in the testis and epididymis were examined with quantitative real-time PCR （qPCR）. Results The organ coefficients of testis were 3.60 ± 0. 41, 3.47 ±0. 41,3.15 ± 0. 46, and 3.14 ± 0. 45 and those of epididymis were 0. 82 ± 0. 14,0. 80 ± 0. 12,0. 70 _± 0. 11, and 0. 68 ± 0. 11 for the mice in the control, 1,2, and 4 mg/L Asz O3 groups,respectively. Compared with the control group, the organ coefficients of testis and epididymis in 2 and 4 mg/L As2 03 groups were decreased significantly （ P 〈 0. 05 ）. The sperm activity in the control group was 97% ,while that in 2 and 4 mg/L As203 groups decreased to 82% and 81% ,re- spectively （P 〈 0. 05 ）. And the sperm malformation ratio of 4 mg/L group was 6. 6% and significantly higher than that in control group （2. 6%, P 〈 0.05 ）. The cells in the seminiferous epithelium section also decreased significantly in 4 mg/L As203 group（P 〈 0. 05 ）. The gene expression of Uty was down-regulated significantly in testis and epididymis in both 2 and 4 mg/L AS203 groups compared with the control. Conclusion Subchronic exposure to arsenic inhibits sperm pro- duction and the male reproductive toxicity induced by As may be closely related to down-regulated expression of Uty.

关 键 词：三氧化二砷(As2O3) 精子发育 Uty基因 雄性生殖毒性 

分 类 号：R994.6[医药卫生—毒理学]