鱼油对PM2.5所致心血管炎性和氧化损伤拮抗作用  

Effect of deep-sea fish oil on PM2.5-induced inflammatory and oxidative stress damage of cardiovascular system in rats

作　　者：李莉珊 马琼锦 裴益玲[1] 杨凌[1] 杨莹莹[1] 蒋蓉芳[1] 宋伟民[1] 

机构地区：[1]复旦大学公共卫生学院公共卫生安全教育部重点实验室,上海200032

出　　处：《中国公共卫生》2015年第6期767-770,共4页Chinese Journal of Public Health

基　　金：国家自然科学基金(81172617)

摘　　要：目的探讨深海鱼油对大气细颗粒物(PM2.5)引起的心血管系统中的炎性损伤和氧化应激损伤影响。方法将SD雄性大鼠随机分为鱼油低、中、高剂量组,鱼油对照组,溶剂对照组(玉米油)和PM2.5染毒组;鱼油组大鼠先进行深海鱼油灌胃28 d,再进行PM2.5气管滴注染毒隔天1次,共3次,PM2.5染毒期间同时进行鱼油灌胃,PM2.5染毒组先正常饲养28 d,再进行PM2.5染毒;取血清检测白细胞介素-6(IL-6)、肿瘤坏死因子α(TNF-α)、超敏C反应蛋白(hs-CRP)、丙二醛(MDA)、总超氧化物歧化酶(T-SOD)、谷胱甘肽(GSH)和谷胱甘肽过氧化物酶(GSH-Px)。结果与溶剂对照组比较,PM2.5染毒组大鼠血清中IL-6、TNF-α、hs-CRP、MDA含量[分别为(24.94±2.06)、(821.45±14.26)ng/m L、(3.10±0.28)mg/L、(15.88±1.41)nmol/m L]升高,GSH、GSH-Px、TSOD活性[(4.62±0.37)μmol/L、(289.28±30.65)活力单位、(239.26±4.97)U/m L]降低(均P<0.05);与PM2.5染毒组比较,鱼油高剂量组大鼠血清中IL-6、TNF-α、hs-CRP、MDA含量[分别为(16.67±0.23)、(73.01±3.80)ng/m L、(1.36±0.22)mg/L、(11.82±0.79)nmol/m L]下降,GSH、GSH-Px、T-SOD活性[(24.05±3.83)μmol/L、(531.33±40.39)活力单位、(273.36±3.92)U/m L]升高,呈剂量效应关系(均P<0.05)。结论深海鱼油对大气PM2.5所致心血管系统炎性反应和氧化应激损伤具有明显拮抗作用。Objective To explore the effect of deep-sea fish oil on inflammatory and oxidativestress damage of car- diovascular system caused by particulate matter ≤ 2. 5 μm in aerodynamic diameter （ PM2. 5 ）. Methods Male Sprague- Dawley rats were divided into low-, medium-, and high-dose deep-sea fish oil groups, deep-sea fish oil control group, sol- vent group（ corn oil）and PM2. 5 exposure group. The PM2. 5 was sampled from ambient air in an urban area of Shanghai city. The rats in deep-sea fish oil groups were administered with deep-sea fish oil by gavage for 28 days and then exposed to PM2. 5 （8.0 mg/kg） with intratracheal instillation once the other day for 3 times. The rats in PM2. 5 exposure group were bred normally for 28 days and then exposed to PM2. 5 by intratracheal instillation. The rats in deep-sea fish oil and solvent control groups were administered with deep-sea fish or corn oil for 35 days, respectively. Serum interleukin 1 -beta （IL-1 β ）, interleukin 6 （IL-6）, tumor necrosis factor alpha （TNF-ct）, high sensitive C-reaction protein （hs-CRP）, malond- ialdehyde （ MDA ）, total superoxide dismutase （T-SOD）, glutathione （ GSH ）, and glutathione peroxidase （GSH-Px） of the rats were detected after the completion of the treatments. Results Compared with the solvent group, the contents of IL-1β （1 155.98 2100.28 ng/ml） ,IL-6（24. 94 ±2.06 ng/ml） ,TNF-ct （821.45 ± 14.26 ng/ml） ,hs-CRP（3. 10 20.28 mg/L） ,and MDA（ 15. 88 21.41 nmol/ml）increased and the contents of GSH（4. 62 20. 37 μmol/L） ,GSH-Px（289. 28 230. 65 U/mi） ,and T-SOD（239.26 ± 4. 97 U/ml） decreased in the rats of PM2. 5 exposure group. Compared with the PM2. 5 exposure group, the contents of IL-6 （ 16. 67 2 0. 23 ng/mL）, TNF-α （ 73.01 ± 3.80 ng/mL）, hs-CRP （ 1.36 ± 0. 22 mg/L）, and MDA （ 11.82 ± 0.79 nmol/rnL） were decreased and GSH （ 24. 05 ± 3.83 μmol/L）, GSH-Px （ 531.33 2 40. 39 U/mL）, and T-SOD（273.36 ± 3.92 U/mL）increased in a dose-re

关 键 词：大气细颗粒物(PM2.5) 深海鱼油 心血管炎性 氧化应激 

分 类 号：R122[医药卫生—环境卫生学]