硒化合物在HepG2细胞中代谢生成硒蛋白效应  

Effects of various selenocompounds on production of SEPP and GPx in HepG2 cells

作　　者：王琴[1] 卢佳希[1] 韩枫[1] 孙丽翠[1] 刘轶群[1] 黄振武[1] 

机构地区：[1]中国疾病预防控制中心营养与健康所营养与代谢研究室,北京100050

出　　处：《中国公共卫生》2015年第6期787-789,共3页Chinese Journal of Public Health

基　　金：国家自然科学基金(81372989);国家自然科学青年基金(31201352);中国营养学会伊利营养与健康研究基金(2013-013)

摘　　要：目的探讨亚硒酸钠(Na2Se O3),硒代蛋氨酸(Se Met)和甲基硒代半胱氨酸(Me Se Cys)3种硒化合物在Hep G2细胞内代谢生成硒蛋白P(SEPP)和谷胱甘肽过氧化物酶(GPx)效应。方法将Hep G2细胞分为对照组、低、中、高剂量硒化合物组(0.01、0.10、1.00μmol/L),作用48、72 h,收集细胞上清液和裂解液;采用双抗夹心酶联免疫吸附试验检测上清中SEPP浓度与裂解液中GPx活力。结果与对照组比较,高剂量Na2Se O3、Se Met、Me Se Cys作用Hep G2细胞48 h时,上清中SEPP浓度[分别为(115.78±1.70)、(140.73±0.97)、(114.92±1.46)pg/m L]均明显升高(P<0.01),裂解液中GPx活力[分别为(490.52±5.40)、(643.26±5.40)、(603.35±6.40)U/L]明显升高(P<0.01);与Na2Se O3、Me Se Cys比较,Se Met作用效果最好(P<0.05);3种硒化合物作用Hep G2细胞48 h与72 h比较,各指标差异均无统计学意义。结论 3种硒化合物中,以Se Met作用于Hep G2细胞后代谢生成SEPP和GPx的效果最明显。Objective To examine stimulative effects of various selenocompounds on the production of selenopro- tein P （SEPP） and glutathione peroxidase （GPx） in HepG2 cells. Methods The cultured HepG2 cells were divided into a control group, low- （ 0.01 μ mol/L）, midium- （ 0. 1 μmol/L）, and high-dose （ 1.00 μ mol/L） groups treated with differ- ent selenocompounds, including sodium selenite （ Na2 SeO3 ） , selenomethionine （ SeMet ）, and methyl selenocysteine （MeSeCys）. Fourty-eight or 72 hours after the treatments,the cell culUlre supernatants and homogenates were collected for the detections of SEPP and GPX with double-antibody sandwich enzyme-linked immuno sorbent assay （ELISA）. Results Compared with the control group ,the concentrations of SEPP （ 115. 78 ± 1.70,140. 73 ± 0. 97, and 114. 92 ± 1.46 pg/mL ） increased significantly in high-dose Na2SeO3 ,SeMet,and MeSeCys groups 48 hours after the treatment（ all P 〈0. 01 ）and those of GPx（490. 52 ±5. 40,643. 26 ±5. 40,and 603. 35 ±6. 40 U/L） also increased significantly（ all P 〈 0. 01 ）. Compared with the other two selenocompounds （Na2 SeO3 and MeSeCys）, SeMet showed the highest stimulative effect （P 〈 0. 05 ）. No significant difference in the stimulative effect was observed between the treatments of 48 and 72 hours. Conclusion Among the three selenocompounds, SeMet has the highest stimulative effect on productions of SEPP and GPx in HepG2 cells.

关 键 词：硒化合物 代谢 硒蛋白P(SEPP) 谷胱甘肽过氧化物酶(GPx) 

分 类 号：R996.1[医药卫生—毒理学]