SDG对去卵巢大鼠心肌缺血再灌注损伤保护作用  

Protective effect of SDG on myocardial ischemia reperfusion injury in ovariectomized rats

作　　者：马敏[1] 张娜娜[1] 师磊[1] 赵志武[1] 李秀花[1] 路健[1] 封启龙[1] 燕炯[1] 

机构地区：[1]山西医科大学公共卫生学院营养与食品卫生学教研室,山西太原030001

出　　处：《中国公共卫生》2015年第6期825-828,共4页Chinese Journal of Public Health

基　　金：山西省基础研究计划项目(2013011049-5)

摘　　要：目的探讨开环异落叶松树脂酚二葡萄糖苷(SDG)对雌性去卵巢大鼠心肌缺血再灌注损伤的保护作用。方法雌性3周龄SD大鼠分为7组:假手术组,去卵巢组、再灌注组、SDG干预组(10、20、50、100 mg/kg),生化方法检测大鼠血清中总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL)、低密度脂蛋白胆固醇(LDL)含量,超氧化物歧化酶(SOD)活力和丙二醛(MDA)含量,心肌组织中caspase-3比活性;用伊文氏蓝和2,3,5-氯化三苯基四氮唑双染色法测定心肌梗死面积;光镜观察心肌细胞病理变化;用DNA原位末端标记法检测心肌细胞凋亡变化。结果与去卵巢组比较,再灌注组大鼠血清中HDL明显降低(P<0.05),TC、TG、LDL明显升高(P<0.05);心肌病理损伤加重、心肌梗死面积增大;血清SOD水平降低、MDA含量增加(P<0.05);心肌细胞凋亡指数增加(P<0.05);与再灌注组比较,50 mg/kg SDG组大鼠血清中HDL[(3.285±0.327)mmol/L]升高、TC、TG、LDL[分别为(5.508±0.479)、(0.535±0.129)、(3.290±0.358)mmol/L]降低(P<0.05);心肌梗死面积缩小、心肌组织病理损伤减轻;血清SOD水平[(285.786±31.287)U/m L]升高,MDA含量[(2.483±0.391)nmol/m L]降低(P<0.05);心肌细胞凋亡指数(17.57±0.84)明显降低(P<0.05);呈剂量效应关系。结论 SDG对去卵巢大鼠心肌缺血再灌注损伤具有保护作用,其机制可能与SDG提高心肌组织抗氧化能力和减少心肌细胞凋亡有关。Objective To explore protective effect of secoisolariciresinol diglucoside （SDG）on ischemia-reperfeu- sion injury in ovariectomized rats. Methods Three-week-old female healthy Sprague-Dawley（SD） rats were divided into 7 groups：sham group, ovariectomized group, ischemia-reperfusion group and different dose of SDG intervention groups （ 10,20,50,100 mg/kg ）. Serum total cholesterol （ TC ）, triglyceride （ TG ）, high-density lipoprotein cholesterol （ HDL ）, low-density lipoprotein cholesterol （ LDL ） content, superoxide dismutase （ SOD ）, malondialdehyde （ MDA ） level and caspase-3 activity in myocardial tissue were detected with biochemical methods. Myocardial infarction area was measured with Even＇s blue and 2,3,5-tripheny-2H-tetrazolianm chloride staining method. Pathological changes of myocardial cells were observed with light microscope. Myocardial cell apoptosis was detected by TdT-mediated d-UTP-biotin nick end-la- beling （TUNEL）. Results Compared with ovariectomized group, serum HDL content of ischemia-reperfusion group de- creased significantly （ P 〈 0. 05 ） ; TC, TG and LDL increased significantly （ P 〈 0. 05 ）. Myocardial pathological damage aggravated and myocardial infarction area increased. Serum SOD, MDA contents reduced （P 〈 0. 05 ）. Myocardial apopto- sis index increased（ P 〈 0. 05 ）. Compared with ischemia-reperfusion group, serum HDL of 50 mg/kg SDG group（ 3. 285 ± 0. 327 retool/L） increased; TC （ 5. 508 ± 0. 479 mmol/L ）, TG （ 0. 535 ± 0. 129 mmol/L ）, and LDL （ 3. 290 ± 0. 358 mmol/L） reduced（ P 〈 0. 05 for all） ;myocardial infarction area reduced and pathological damage of myocardial tissue al- leviated;serum levels of SOD（285. 786 ±31. 287 U/ml） ,MDA（2. 483 ±0. 391 nmol/mL）reduced （P 〈0. 05） and my- ocardial apoptosis index（ 17.57 ± 0. 84 ） decreased significantly （ P 〈 0. 05 ）. All of the changes showed dose-effect rela- tionships. Conclusion SDG has a protective effect on my

关 键 词：开环异落叶松树脂酚二葡萄糖苷(SDG) 卵巢切除术 缺血再灌注 抗氧化 凋亡 植物性雌激素 

分 类 号：R181.24[医药卫生—流行病学]