237例血培养阳性结果分析  

Analysis of 237 positive results from blood cultures

作　　者：陈梅莲[1] 邓任堂[1] 李柳燕[1] 李金洳 付文金[1] 

机构地区：[1]广东医学院附属厚街医院检验科,广东东莞523945

出　　处：《实验与检验医学》2015年第3期293-295,299,共4页Experimental and Laboratory Medicine

摘　　要：目的对血培养病原菌的种类分布和耐药情况进行回顾性分析,为临床用药提供依据。方法采用Versa TREK全自动血培养仪及配套血培养瓶连续监测培养细菌,阳性瓶及时转种。用Micro Scan A/S-4微生物分析仪及配套试剂对分离菌进行鉴定和药敏试验。结果 2460例血培养标本中共分离出237株病原菌,居前5位的病原菌依次为凝固酶阴性葡萄球菌、大肠埃希菌、肺炎克雷伯菌、金黄色葡萄球菌和肠球菌。临床分布以ICU、普外科及呼吸内科为主。药敏结果显示:革兰阴性杆菌对亚胺培南高度敏感,未发现耐万古霉素和利奈唑胺的革兰阳性球菌。结论及时了解血培养结果可以为临床抗菌治疗提供依据,对提高治愈率有重要的意义。Objective To investigate the strains distribution and resistance characteristics of pathogenic bacteria isolated from blood culture and provide basis for clinical medication. Methods Blood samples were cultivated and continuously monitored by VersaTREK automated blood culture system with the matching blood culture bottles,and the positive bottles were transferred timely. The identification of bacteria and susceptibility test were performed by MicroScan A/S-4 bacterial automatic analyzer. Results A total of 237 strains of pathogenic bacteria were isolated from 2460 blood culture samples. The top five strains included CNS, Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus and Enterococcus. Positive pathogens were mainly distributed in ICU, general surgical department and respiratory medicine department. Susceptibility tests showed that Gram negative bacilli were highly sensitive to imipenem and Gram positive cocci had no resistance to vancomycin and linezolid. Conclusion It is important to comprehend the blood culture results promptly for the selection of antimicrobial agents and has great significance to improve cure rate.

关 键 词：血培养 病原菌 耐药性 

分 类 号：R446.5[医药卫生—诊断学] Q939.92[医药卫生—临床医学]