人胚胎干细胞向嗜酸性粒细胞高效定向诱导分化模型的建立  

Establishment of an efficient eosinophils directional differentiation model from human embryonic stem cells

作　　者：潘旭[1] 杨文钰[2] 孙文翠[1] 毛斌[1] 郁金凤 路旭琳 黄淑[1] 赖默温 周涯[1] 邹庆[3] 李晴[1] 曾宪波[1] 周家喜[2] 马峰[1,2] 

机构地区：[1]中国医学科学院北京协和医学院输血研究所,四川成都610052 [2]中国医学科学院北京协和医学院血液学研究所实验血液学国家重点实验室 [3]四川新生命细胞科技股份有限公司

出　　处：《中国输血杂志》2015年第5期479-482,共4页Chinese Journal of Blood Transfusion

基　　金：国家自然科学基金(NSFC81170466);国家重点基础研究发展计划(973计划)资助项(2015CB964902)

摘　　要：目的建立从人胚胎干细胞向成熟嗜酸性粒细胞定向诱导分化的高效体外培养模型。方法采用人胚胎干细胞与小鼠主动脉-性腺-中肾(m AGM)基质细胞共培养的方法,进行早期造血干细胞诱导分化,将诱导分化的造血干细胞进行悬浮培养,在含有血清的培养基中,分阶段添加不同细胞因子组合进行成熟嗜酸性粒细胞的定向分化和增殖。结果获得从CD34+CD45+细胞扩增了约651倍,且纯度高达95%以上,CD88+Siglec-8+EPO+的成熟嗜酸性粒细胞。结论本研究建立了1种人胚胎干细胞高效定向分化为成熟嗜酸性粒细胞的模型,为进一步深入研究嗜酸性粒细胞的早期发育调控和相关疾病的发生机制奠定了基础。Objective To establish a model for inducing directional differentiation of human embryonic stem cells into mature eosinophils in vitro. Methods Human embryonic stem cells and mAGM-S3 （a routine AGM-derived stromal cell line） were first co-cultured to induce the development into early hematopoietic stem cells, then were induced into mature eosinophils in suspension culture with different cytokine combinations which were specific for proliferation and maturation ＂of human eesinophils. Results CD34 ＋ CD45 ＋ cells were induced to differentiate 651 times into CD88 ＋ Siglec-8 ＋ EPO ＋ mature eesinophils with 95% purity. Conclusion This study established an efficient model in which human embryonic stem cells undergo directional differentiation into mature eosinophils which helps lay a foundation for further studies on mechanisms that control early developmental regulation of human eosinophils and related diseases.

关 键 词：人胚胎干细胞 嗜酸性粒细胞 定向诱导分化 

分 类 号：R457.7[医药卫生—治疗学]