一种引起大豆根腐病的尖镰孢致病性苗期鉴定方法  

A Method to Determine Pathogenicity of Fusarium oxysporum Causing Root Rot of Soybean at Seedling Stage

作　　者：王春玲[1] 耿肖兵 黄铭慧 孙丽萍[1] 李永刚[1,2] 

机构地区：[1]东北农业大学农学院,哈尔滨150030 [2]东北农业大学寒地作物品种改良与生理生态重点开放实验室,哈尔滨150030

出　　处：《吉林农业科学》2015年第3期69-72,共4页Journal of　Jilin Agricultural Sciences

基　　金：东北农业大学大豆生物学教育部重点实验室开放基金资助(SB12B01)

摘　　要：通过试验研究,建立了一个科学、快速和准确的尖镰孢侵染大豆所致根腐病苗期鉴定方法。利用该鉴定方法,分析了6株尖镰孢与11个大豆品种的互作。结果表明不同品种间抗性差异较大,如绥农28与菌株K31互作病指为15.24,而与菌株YA25互作病指为44.90;东农54与菌株M63互作病指为19.31,而与菌株H1互作病指为71.43。通过应用该鉴定方法,证明其适用于大批量、快速和准确地进行尖镰孢与大豆品种(品系)互作中的尖镰孢致病性测定及抗源筛选。A method was developed in this study to fast and accurately determine pathogenicity of Fusarium oxysporum causing root rot of soybean at seedling stage. The method was used to study interactions between 6 stains of F. oxysporum and 11 soybean cultivars. Results showed that there were significant differences among the cuhivars in resistance to the disease. Mean disease indices were 15.24, 44.90, 19.31, or 71.43 in the interactions between Su- inong 28 and strain K31, Suinong 28 and strain YA25, Dongnong 54 and strain M63, and Dongnong 54 and strain HI, respectively. The results indicated that this method could be applicable to large-scale, fast and accurate deter- mination of pathogenicity of F. oxysporum and screening of host resistance in interactions between the pathogen and soybean cuhivars or lines.

关 键 词：大豆根腐病 尖镰孢 分级标准 症状 鉴定方法 

分 类 号：S435.2[农业科学—农业昆虫与害虫防治]