不同玉米品种抗感粗缩病与过氧化物酶关系的研究  

Studies of the Relationship between Resistance to Maize Rough Dwarf Virus(MRDV) and POD in Different Maize Varieties

作　　者：穆西玉 张海艳[1,2] 

机构地区：[1]青岛农业大学农学与植物保护学院/山东省旱作农业技术重点实验室,山东青岛266109 [2]山东省小麦玉米周年高产高效生产协同创新中心,山东泰安271018

出　　处：《吉林农业科学》2015年第3期73-75,102,共4页Journal of　Jilin Agricultural Sciences

基　　金：青岛市科技计划项目[11-2-4-5-(8)-jch];山东省科技发展计划(2014GNC111001);山东省农业良种工程泰山学者种业人才团队支撑计划培养对象项目(20140701);山东省旱地作物水分高效利用科研创新团队(6212n2)

摘　　要：以6个对玉米粗缩病(MRDV)表现不同抗性的玉米品种为材料,分析抽雄期健株和病株叶片过氧化物酶(POD)活性及其同工酶酶谱的差异。结果表明,品种间比较,POD活性表现为感病品种>中抗品种>抗病品种;感病后,POD活性显著增加,且增加幅度表现为抗性品种>中抗品种>感病品种。说明品种抗性越强,POD活性越低,感病后POD活性增加幅度越大。不同抗性玉米品种POD同工酶的表达存在一定的差异,Rf0.93酶带的表达与品种的抗病性有关,感病后Rf 0.28酶带表达强度的增强与品种诱导抗病性有关。因此,品种抗性越强,Rf 0.93酶带的表达越强,感病后Rf 0.28酶带表达的增强幅度越大。Six maize （Zea mays L.） varieties with different resistance to maize rough dwarf virus （MRDV） were used to analyze POD activity and isozyme pattern of healthy and diseased leaves in the tasseling stage. The results indicated that POD activity appeared susceptible varieties 〉 moderate resistant varieties 〉 resistant varieties. POD activity increased after the plants were infected by MRDV and the increasing extent showed resistant varieties 〉 moderate resistant varieties 〉 susceptible varieties. So, the stronger resistance, the less POD activity, and the greater increase of POD activity. POD isozyme was different in resistant and susceptible varieties. The band of Rf 0.93 was related to the resistance to MRDV. The increasing extent of Rf 0.28 expression after MRDV infection was related to the inductive resistance. Therefore, the stronger resistance, the greater Rf 0.93 expression, the greater increasing ex- tent of Rf 0.28 expression after MRDV infection.

关 键 词：玉米 粗缩病 POD活性 POD同工酶 

分 类 号：S435.131[农业科学—农业昆虫与害虫防治]