接种AMF对不同葡萄品种菌丝酶活性及无机营养吸收的影响  

Effect of Inoculation with AMF on Hyphal Enzyme Activity and Inorganic Nutrient Absorption of Different Grape Cultivars

作　　者：张永福[1] 任禛[1] 韩丽[1] 彭声静[1] 陈泽斌[1] 牛燕芬[1] 

机构地区：[1]昆明学院农学院,昆明650214

出　　处：《吉林农业科学》2015年第3期97-102,共6页Journal of　Jilin Agricultural Sciences

基　　金：云南省教育厅科学研究基金项目(2012Z097);昆明学院引进人才科研项目(YJL11030;YJL12002;YJL12007)

摘　　要：本研究选用6个不同生长势的葡萄品种扦插苗为材料,通过盆栽接种试验,探讨摩西球囊霉(Glomus mosseae,Gm)对参试材料丛枝菌根真菌(AMF)侵染率及菌丝酶活性的影响,并分析AMF对试材无机营养吸收能力的影响。结果表明,接种Gm能够显著提高参试材料的AMF侵染率,且强生长势品种的侵染率显著高于弱生长势品种;接种后能够不同程度地提高试材的菌丝酶(ALP和SDH)活性,6个品种的菌丝酶活性大小依次为玫瑰蜜>早黑蜜>水晶>维拉莎>京秀>红地球;丛枝菌根的形成对各试材无机营养的吸收均有影响;其中,对促进大量元素吸收的效果优于微量元素,使试材根、茎、叶中的大量元素含量均显著高于CK,但却出现部分试材的微量元素含量低于CK的情况,其中以茎最为典型;接种对不同品种无机营养吸收的贡献率表现各异,相对而言弱生长势品种的贡献率大于强生长势品种。Through potted inoculation experiment, grape cutting seedlings of six cuhivars with different growth potential were selected as plant material to explore the effect of Glomus mosseae （Gm） on arbuscular mycorrhizal fungus （AMF） infection rate and hyphal enzyme activity, and to analyze the effect of AMF on inorganic nutrient ab- sorption capacity of the materials. The results showed that Gm vaccination could significantly improve AMF coloni- zation of tested cuhivars and infection rates of strong growth potential cultivars were significantly higher than the weak growth potential cuhivars. Inoculation improved hyphal enzymes （ALP and SDH） activity of tested material in following order： Meiguimi 〉 Zaoheimi 〉 Shuijing 〉 Weilasha 〉 Jingxiu 〉 Red globe. Inorganic nutrients absorption of tested grape cuhivars were affected by the formation of AM, and absorption of major elements increased more than trace elements. Content of major elements in root, stem, leaf were significantly higher than CK, whereas trace elements in parts of the tested materials were lower than CK, especially in stem. Contribution rate of Gm to the ab- sorption of inorganic nutrients behaved differently in different cuhivars, contribution rate to relatively weak potential cuhivars were higher than the stronger growth potential cuhivars.

关 键 词：葡萄 丛枝菌根 侵染率 菌丝酶活性 无机营养 

分 类 号：S663.1[农业科学—果树学]