悬浮红细胞对异体T淋巴细胞增殖的影响及机制  

Effect of red blood suspension on proliferation of allogene T lymphocytes and it's mechanisms

作　　者：赵学涛[1] 杨从容[1] 任晓亮[1] 李明[1] 解晓元[1] 巨红妹[1] 

机构地区：[1]河北医科大学第四医院,河北石家庄050011

出　　处：《现代中西医结合杂志》2015年第19期2071-2073,共3页Modern Journal of Integrated Traditional Chinese and Western Medicine

摘　　要：目的体外观察悬浮红细胞对异体T淋巴细胞增殖的影响并探讨其可能机制。方法采用Ficoll低密度离心法和免疫磁珠法从人外周血中分离纯化出CD3+T淋巴细胞,给予CD3/CD28抗体共刺激,并分别与异体非去白悬浮红细胞或去白悬浮红细胞混合培养72 h。采用H3-胸腺嘧啶核苷掺入法检测各组T淋巴细胞增殖的情况。采用流式细胞仪检测各组培养体系中CD4+/CD8+及CD4+CD25+/CD4+细胞亚群比例。结果与阴性对照组(细胞培养液组)对比,非去白悬浮红细胞组和去白悬浮红细胞组T淋巴细胞增殖明显受到抑制,CD4+/CD8+细胞亚群比例下降(P<0.01),而CD4+CD25+/CD4+细胞亚群比例明显增加(P<0.01)。与去白悬浮红细胞相比,非去白悬浮红细胞的作用更明显(P<0.05)。结论悬浮红细胞可能通过上调CD4+CD25+调节性T细胞比例抑制异体T淋巴细胞增殖,而这种作用可能主要取决于悬浮红细胞内残留的白细胞。Objective It is to explore the effect of red blood suspension on proliferation of allogene T lymphocytes in vitro and to explore its possible mechanisms. Methods CD3^＋ T cells from human peripheral blood were isolated and purified by Ficoll density gradient centrifugation combined with adherence MACS selection. Purifed human T cells were stimulated with anti- CD3/anti - CD28 and then exposed to allogene leukocyte-containing red blood suspension or leukocyte-depleted red blood suspension for 72 h. T cell proliferation was determined by 3H - thymidine. The ratios of CD4^ ＋/CD8 ^＋ and CD4^＋ CD25^＋/CD4^ ＋ subgroup from different co-culture systems were detected by flow cytometry. Results Compared with that of negative group （culture media） , the proliferations of allogene T lymphocytes were greatly suppressed,the ratio of CD4^＋/CD8^＋ subgroup decreased, but the ratio of CD4^＋ CD25^＋/CD4^ ＋ subgroup increased in leukocyte-containing group and leukocyte-depleted group （P 〈 0.01 ） , while the influences were more obvious in leukocyte-containing group （P 〈 0.05 ）. Conclusion Red blood suspension could suppress significantly allogene T cell proliferation by up regulation of CD4^＋ CD25^ ＋ regulatory T （Treg） cells. This regulation, to a large extent, depending on the the remaining white blood cells in red blood suspension.

关 键 词：悬浮红细胞 异体T淋巴细胞 CD4^+/CD8^+细胞亚群 CD4^+CD25^+TREG 

分 类 号：R392.12[医药卫生—免疫学]