基于苍术属植物水溶性成分HPLC指纹图谱的化学亲缘关系研究  

Analysis on Chemical Genetic Relationship of Atractylodes Based on HPLC Fingerprint Spectra of Water-soluble Components

作　　者：芮梦珏 欧阳臻[1] 彭华胜[2,3] 赵明[1] 叶丹[1] 邵扬[1] 江珊[1] 杨淑芳[1] 袁婷[1] 

机构地区：[1]江苏大学药学院,江苏镇江212013 [2]安徽中医药大学药学院,安徽合肥230031 [3]中国中医研究院中药资源中心,北京100700

出　　处：《中国野生植物资源》2015年第3期1-6,11,共7页Chinese Wild Plant Resources

基　　金：国家自然基金项目(81072985/H2801);江苏大学第十三批学生科研立项一般项目(13A156)

摘　　要：采用高效液相色谱法建立不同产地的苍术属植物水提物不同极性部位的指纹图谱,并运用"中药色谱指纹图谱相似度评价系统"及SPSS软件对其进行相似度评价及综合聚类分析,以探讨苍术属7种植物的种间亲缘关系。结果显示:茅苍术与罗田苍术聚为一类,北苍术与朝鲜苍术、部分茅苍术聚为一类,关苍术与白术聚为一类,鄂西苍术单独聚为一类,该聚类分析结果与以挥发性成分为指标的聚类分析结果一致。苍术属植物水溶性成分HPLC指纹图谱的建立及聚类分析可为苍术属植物的分类,寻找苍术属植物亲缘关系提供可靠依据。High-performance liquid chromatography was used to establish the chromatographic fingerprints of the different polar extracts obtained from Atractylodes. Traditional Chinese Medicine （TCM） chromatographic fingerprint similarity evaluation system and SPSS was used to analyze the similarity eval- uation and cluster analysis, in order to probe the genetic relationship of Atractylodes. Cluster analysis out- comes demonstrated that Atractylodes can be divided into four categories： Atractylodes lancea （Thunb.） DC. and Atractylodes lancea subsp, luotianensis S.L. Hu et X. F. Feng; Atractylodes macrocephala Koidz. and Atractylodes japonica Koidz. ; part of Atractylodes lancea （ Thunb. ） DC. and Atractylodes coreana （ Nakai ） Kitam. and Atractylodes chinensis （ DC. ） Koidz ; Atractylodes carlinoides （ Hand. - Mazz. ） Kitam. The result obtained from the cluster analysis is similar to the one obtained when volatile oil components are used as indicator. Establishing the chromatographic fingerprints of the water-soluble components from Atractylodes provided reliable and solid evidence for Atractylodes classification and the eluci- dation of the genetic relationship of Atractylodes.

关 键 词：苍术属 亲缘关系 指纹图谱 综合聚类分析 

分 类 号：R284[医药卫生—中药学]