真菌性阴道炎患者的假丝酵母菌感染及耐药性研究  

The infection and drug-resistance of Candidas in patients with colpitismycotica

作　　者：杨柳[1] 李鹏宇[1] 王智[1] 

机构地区：[1]武警北京总队医院检验科,北京100027

出　　处：《检验医学与临床》2015年第12期1730-1732,共3页Laboratory Medicine and Clinic

摘　　要：目的探讨真菌性阴道炎假丝酵母菌的感染及其耐药性。方法选取2011年11月至2014年12月该院128例真菌性阴道炎患者,分离其阴道分泌物中的假丝酵母菌并进行耐药性检测;采用实时定量-聚合酶链式反应(RT-PCR)检测和比较咪康唑敏感株、中介株和耐药株的多药耐药基因MDR1、FLU1、CDR1的表达水平。结果128例患者共分离出132株(6种),84例(63.64%)检测出假丝酵母菌,4例(3.13%)患者有2种菌株的重叠感染。特比萘芬的耐药率为44.70%,制霉菌素的耐药率为0.00%,敏感率为96.21%。3组菌株的MDR1和FLU1表达水平差异有统计学意义(P<0.05);但CDR1表达水平差异无统计学意义(P>0.05)。结论真菌性阴道炎的假丝酵母菌感染呈现复杂化趋势,临床应根据患者具体情况选择抗菌药物。Objective To analyze the infection and drug-resistance of Candidasin patients with colpitis mycotica.Methods From November 2011 to October 2014,128 patients with colpitismycotica were chosen as study objects.The candidas in vaginal fluid of patients were isolated,and the expression level of MDR1,FLU1 and CDR1were compared among miconazole-sensitive,miconazole-medium and miconazole-resistant strains.Results In 128 patients,4patients（3.13%）were infected with two kinds of Candidas,and 132strains（6kinds）were isolated and 84strains（63.64%）were Candida albicans.The resistant rate of terbinafine was 44.70%;the resistant rate of naystatin was0.00% and its sensitive rate was 96.21%.The expression level of MDR1,FLU1 had statistical difference among miconazole-sensitive,miconazole-medium and miconazole-resistant strains（P〈0.05）,and that of CDR1 had no statistical difference（P〉0.05）.Conclusion The infection of Candidas in patients with colpitis has become more complexing,and the use of antifungals should be chosen according to condition of patients.

关 键 词：真菌性阴道炎 假丝酵母菌 耐药性 

分 类 号：R446.5[医药卫生—诊断学]