脂肪组织AR1 mRNA表达和PDK1蛋白活性在PCOS胰岛素抵抗中的作用  

AdipoR1 mRNA and 3-phosphoinositide-dependent protein kinase-1 activity in adipose tissue of PCOS

作　　者：王竹晨[1,2] 甘辉梅[2] 胡钶[3] 李丽文[2] 顾熊飞[4] 杨冬梓[5] 邝健全[5] 

机构地区：[1]香港大学深圳医院生殖医学中心,广东深圳518053 [2]深圳市第二人民医院生殖医学科 [3]深圳市宝安区人民医院普外科 [4]中山大学北校区生物化学教研室 [5]中山大学孙逸仙纪念医院妇产科

出　　处：《临床医学》2015年第5期1-4,共4页Clinical Medicine

基　　金：深圳市科技研发资金基础研究计划(JC201105170658A)

摘　　要：目的探讨多囊卵巢综合征(PCOS)脂肪组织脂联素受体AdipoR1(AR1)mRNA表达和3-磷酸肌醇依赖性蛋白激酶-1(PDK1/PDPK1)的蛋白活性在PCOS发病中的作用。方法 PCOS患者和对照者根据体质量指数(BMI)分为PCOS肥胖组、PCOS非肥胖组、肥胖对照组和非肥胖对照组,每组12例,采用RT-PCR技术结合内对照,半定量检测PCOS及其对照组脂肪组织AR1 mRNA的表达,采用脂肪组织蛋白质的提取、SDS-Page、Western blot和增强化学发光蛋白免疫印迹法及图像分析半定量检测脂肪组织PDK1活性。结果脂肪组织中AR1 mRNA的表达量在PCOS肥胖组(0.49±0.13)和PCOS非肥胖组(0.74±0.14)均显著低于非肥胖对照组(0.88±0.15),且以PCOS肥胖组降低更为明显(P<0.001),PCOS肥胖组与肥胖对照组(0.60±0.17)相比差异未见统计学意义(P>0.05)。PDK1蛋白活性在PCOS肥胖组为(55.16±24.37)%,较非肥胖对照组的(84.33±15.54)%明显降低(P<0.001);肥胖对照组为(45.95±22.18)%,PCOS非肥胖组为(71.27±26.42)%,均较非肥胖对照组明显降低(P<0.001和P<0.05),PCOS肥胖组和肥胖对照组之间以及PCOS肥胖组和PCOS非肥胖组之间比较差异均未见统计学意义(P>0.05)。结论 PCOS两组脂肪组织AR1mRNA的表达均较非肥胖对照组降低,且以PCOS肥胖组降低更为明显,PCOS肥胖组和PCOS非肥胖组脂肪组织PDK1活性均较非肥胖对照组明显减弱,低水平的AR1可能通过下调PDK1抑制胰岛素受体后的信号传导,并与PCOS胰岛素抵抗的发生有关。Objective To study adipoR1 （ AR1 ） mRNA and 3-phosphoinositide-dependent protein kinase-1 （ PDK1 ） activity of adipose tissue in potycystic ovary syndrome （PCOS） to approach the mechanism of insulin resistance （IR） in PCOS at the tis- sular and cellular level. Methods Patients with PCOS and healthy controls were divided into obese PCOS group, non-obese P- COS group, obese control group and non-obese control group according to their body mass index （BMI） with 12 cases in each group. AR1 mRNA were studied with semi-quantitative RT-PCR technique with inner-control, PDK1 activity of adipose tissue were studied with immunoprecipitation, western-blot, ECL immunoblotting. Results The expression of AR1 mRNA in adipose of the obese PCOS group （0. 49±0. 13） and the non obese PCOS group （0. 74 ±0. 14） were higher than that in the non-obese control group （0. 88±0. 15）, and the obese PCOS group increased more significantly （P 〈 0. 001 ）. The difference between the obese PCOS group and the obese control group （0. 60 ±0. 17） had no significant difference （P 〉0. 05）. PDK1 activity of adi- pose tissue in the obese PCOS group [ （55.16± 24. 37 ） %, P 〈 0. 001 ], the obese control group [ （45.95 ±22. 18 ） %, P 〈 0. 001 ] and in the non obese PCOS group [ （71.27 ± 26. 42） %, P 〈 0. 05 ] was lower than that in the non-obese control group [ （84. 33±15.54）% ]. The differences between the obese PCOS group and the obese control group and the two PCOS groups were not significant （P 〉 0. 05）. Conclusion The expression of AR1 mRNA in adipose tissue of PCOS patient is abnormally decreased. The lower level of AR1 can down-regulate PDK1 activity in PCOS women which may lead to the disorder of insulin- signaling transduction downstream of insulin receptor and is correlated with IR.

关 键 词：多囊卵巢综合征 胰岛素抵抗 脂肪组织 胰岛素受体底物I PI3K蛋白活性 

分 类 号：R711.75[医药卫生—妇产科学]