对两个分离自海南尖峰岭的木本豆科植物根瘤菌菌株的多相鉴定  

Polyphasic Identification of Two Rhizobium Strains from Woody Legumes in Jianfengling of Hainan Province

作　　者：程涛[1,2] 焦如珍[1,2] 高俊莲 

机构地区：[1]中国林业科学研究院林业研究所,北京100091 [2]国家林业局林木培育重点实验室,北京100091 [3]北京市农林科学研究院生物技术研究中心,北京100097

出　　处：《林业科学研究》2015年第3期380-386,共7页Forest Research

基　　金：948项目(2013-4-54);林业科技推广项目([2012]8号)

摘　　要：对分离自海南尖峰岭自然保护区木本豆科植物的2个根瘤菌菌株RIF200835和RIF200845进行了鉴定。这两个菌株的16S r DNA序列同模式菌株Rhizobium miluonense CCUAU 41251T的相似度分别为98.28%和98.51%。对这两个菌株进行全细胞脂肪酸组分和全细胞醌组分的分析结果表明,菌株RIF200835和RIF200845在组分上与模式菌株较为一致,但在含量上稍有差异。RIF200835和RIF200845这两个菌株的DNA G+C mol%含量分别为63.68%和60.56%,介于根瘤菌G+Cmol%含量范围(57%65%)之内。这两个菌株的看家基因atp D、gln II、rec A和rpo B,不同于模式菌株,而独立聚成一个分支。研究结果表明,菌株RIF200835和RIF200845可能为根瘤菌属Rhizobium中的新种。Two Rhizobium strains RIF200835 and RIF200845 isolated from woody legumes in Jianfengling National Nature Reserve, Hainan province, were analyzed by molecular methods. Their 16S rDNA sequences showed 98.28% and 98.51% of similality to R. miluonense CCUAU 41251T respectively. The two strains were also investi- gated for compositions of fatty acid and quinone. The results showed that they were basically similar to the model strain in content but slightly different in components. The DNA G ＋ C contents of the two strains were 63.68% and 60.56%, falling in the range of 57% - 65% of the and rpoB） formed a clade seperating from the model genus of Rhizobia. Their housekeep genes （atpD, glnlI, recA strain R. miluonense CCUAU 41251T. The results showed that the two strains might be new species of Rhizobium.

关 键 词：根瘤菌 全细胞脂肪酸组分 全细胞醌组分 看家基因 DNA G+C mol%含量 

分 类 号：S714.3[农业科学—林学]