杜仲基因组微卫星特征及SSR标记开发  

Characterization of Genomic Microsatellites and Development of SSR Markers of Eucommia ulmoides

作　　者：吴敏[1,2] 杜红岩[1,2,3] 乌云塔娜[1,2,3] 刘攀峰[1,2,3] 荆腾[1,2] 

机构地区：[1]中国林业科学研究院经济林研究开发中心,河南郑州450003 [2]国家林业局杜仲工程技术研究中心,河南郑州450003 [3]国家林业局泡桐研究开发中心,河南郑州450003

出　　处：《林业科学研究》2015年第3期387-393,共7页Forest Research

基　　金：国家林业公益性行业科研专项:杜仲育种群体建立与综合利用技术研究(201004029)

摘　　要：为全面解读杜仲的遗传背景,本研究在基因组测序(数据未公布)的基础上,通过MISA软件搜索杜仲基因组(26 947/854 758 160 bp)中的完整型(1 7核苷酸重复)及复合型SSR序列,共查找出25 694个Scaffolds含有488592个SSR位点,占总Scaffolds的95.3%。从SSR位点分布密度上讲,平均每1 749 bp出现1个微卫星,其中单核苷酸重复单元的SSR含量最多,约占总数的54.34%,其次为二核苷酸(20.47%),而复合模式、三核苷酸、四核苷酸、五核苷酸、六核苷酸和七核苷酸重复分别占20.29%、23.89%、0.77%、0.13%、0.10%和0.01%,并发现SSR中均以含A、T的重复类型占主导地位。根据SSR位点设计并合成引物290对,162对SSR引物扩增出清晰的目的条带,其中16对引物多态性高、稳定性好,在8份杜仲资源中共检测到84个等位基因,平均每个SSR位点检测到5.25个等位基因。本研究对于进一步利用SSR分子标记分析杜仲遗传多样性、遗传图谱构建、杜仲雌雄株早期鉴定等具有较高的应用价值。Based on genome sequencing of Eucommia ulmoides, the perfect SSR （1 - 7 nucleotide repeats） and compound SSR sequences were searched from Eucommia genome （26,947/854,758,160 bp） by using MISA. 488, 592 SSR loci were found from 25,694 Scaffolds, accounting for 95.3% of the total Scaffolds. The results indicated that every 1 749 bp contained one SSR microsatellite on the average. The SSRs which had mononucleotide repeats were the most of all, accounting for 54.34%, and the dinucleotide repeats accounted for 20.47%. The percentage of compound SSR, trinucleotide repeats, tetranucleotide repeats, pentanucleotide repeats, hexanucleotide repeats, and heptanucleotides repeats were 20.29%, 23.89%, 0.77%, 0. 13%, 0. 10% and 0.01%, respectively. Meanwhile, the A/T repeats were predominant type among these SSRs. A total of 290 pairs of primer was designed and synthesized. The primers were verified by using 8 Eucommia clones, among which 162 pairs of primers were able to amplify products and 16 pairs of them were polymorphic and stable. 84 alleles were detected in these Eucommia clones, （5.25 alleles per SSR locus in average）. The results are applicable for analyzing genetic diversity constructing genetic map, and identifying early sex status by using SSR molecular markers on Eucommia ulmoides.

关 键 词：杜仲 基因组 标记开发 多态性 

分 类 号：S718.46[农业科学—林学]