真武汤对心力衰竭大鼠血清MMP-9及TIMP-1水平的影响  

Effects of Zhenwu Decoction on the Levels of the MMP- 9 and TIMP- 1 in the Serum of Rats with Heart Failure

作　　者：刘洋[1] 李文杰[2] 张洪霞[1] 

机构地区：[1]辽宁中医药大学,辽宁沈阳110847 [2]辽宁中医药大学附属医院,辽宁沈阳110032

出　　处：《辽宁中医杂志》2015年第6期1346-1348,共3页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(81173184)

摘　　要：目的:观察真武汤对心力衰竭大鼠血清基质金属蛋白酶-9(MMP-9)及机制金属蛋白酶抑制剂-1(TIMP-1)的影响,探讨其对治疗心力衰竭的作用机制。方法:本实验通过结扎Wistar雄性大鼠左冠状动脉前降支,配合力竭式游泳造成心肌梗死后心力衰竭模型,分为中药组(真武汤高剂量组、中剂量组、低剂量组)、西药(卡托普利)组、模型组,空白对照组(正常组),药物干预15 d。通过酶联免疫法(ELISA)检测血清中MMP-9、TIMP-1及BNP的变化情况。结果:与正常组相比,模型组BNP、MMP-9水平显著升高(P<0.01),TIMP-1水平显著下降(P<0.01);与模型组相比,各组BNP水平均显著下降(P<0.01),中剂量、高剂量组MMP-9明显降低(P<0.05,P<0.01),TIMP-1水平明显升高(P<0.01);与西药组相比,高剂量组BNP、MMP-9水平显著降低(P<0.05,P<0.01),TIMP-1水平显著升高(P<0.01)。结论:真武汤通过调控MMP-9和TIMP-l的水平,可能是其逆转心室重塑、治疗CHF的作用机制之一。Objective ：To observe the effects of Zhenwu Decoction on the matrix metalloproteinases -9 （MMP- 9） and tissue inhibitor of metalloproteinases - 1 （TIMP - 1 ） in the serum of rats with heart failure. Methods ： Wistar male rats using ligation of the left anterior descending coronary artery in combination with exhausted swimming, the establishment of heart failure after myo-cardial infarction animal models were divided into the Chinese medicine group （ low -~ dose group, moderate - dose group, high - dose group）, western medicine group, model group, a separate blank control group （normal group）, 15 days of drug intervention;by enzyme -linked immunosorbent assay（ELISA） to detect the changes of MMP -9 ,TIMP -1 and BNP. Results：Compared with the normal group, the levels of BNP and MMP - 9 increased distinctly in model group （ P 〈 0. 01 ） ; the level of TIMP - 1 decreased distinctly（ P 〈 0. 01 ）. Compared with the model group, the level of BNP decreased distinctly in each group （ P 〈 0. 01 ） ; the level of MMP-9 decreased distinctly in moderate- dose group and high- dose group（P 〈0. 05 ,P 〈0.01 ） ;the level of TIMP- 1 in- creased distinctly（ P 〈 0. 01 ）. Compared with the western group, the levels of BNP and MMP - 9 decreased distinctly in high - dose group（ P 〈 0. 05, P 〈 0. 01 ） ; the level of TIMP - 1 increased distinctly（ P 〈 0. 01 ）. Conclusion ： Zhenwu Decoction can adjust the levels of MMP - 9 and TIMP - 1 which may be one of the mechanisms of reversing myocardial model to treat heart failure.

关 键 词：真武汤 心力衰竭 MMP-9 TIMP-1 BNP 

分 类 号：R720.597[医药卫生—急诊医学]